Department of Pharmaceutical Sciences, University of Perugia, Via Romana, 06126 Perugia, Italy.
Int J Mol Sci. 2023 Feb 19;24(4):4157. doi: 10.3390/ijms24044157.
The intestinal epithelium constitutes a selectively permeable barrier between the internal and external environment that allows the absorption of nutrients, electrolytes, and water, as well as an effective defense against intraluminal bacteria, toxins, and potentially antigenic material. Experimental evidence suggest that intestinal inflammation is critically dependent on an imbalance of homeostasis between the gut microbiota and the mucosal immune system. In this context, mast cells play a crucial role. The intake of specific probiotic strains can prevent the development of gut inflammatory markers and activation of the immune system. Here, the effect of a probiotic formulation containing LR 32, BL04, and BB 536 on intestinal epithelial cells and mast cells was investigated. To mimic the natural host compartmentalization, Transwell co-culture models were set up. Co-cultures of intestinal epithelial cells interfaced with the human mast cell line HMC-1.2 in the basolateral chamber were challenged with lipopolysaccharide (LPS), and then treated with probiotics. In the HT29/HMC-1.2 co-culture, the probiotic formulation was able to counteract the LPS-induced release of interleukin 6 from HMC-1.2, and was effective in preserving the epithelial barrier integrity in the HT29/Caco-2/ HMC-1.2 co-culture. The results suggest the potential therapeutic effect of the probiotic formulation.
肠上皮构成了内环境和外环境之间具有选择性渗透性的屏障，允许营养物质、电解质和水的吸收，以及对腔内细菌、毒素和潜在抗原物质的有效防御。实验证据表明，肠道炎症的发生与肠道微生物群和黏膜免疫系统之间的稳态失衡密切相关。在这种情况下，肥大细胞起着至关重要的作用。摄入特定的益生菌菌株可以预防肠道炎症标志物的发展和免疫系统的激活。在这里，研究了含有 LR32、BL04 和 BB536 的益生菌配方对肠上皮细胞和肥大细胞的影响。为了模拟天然的宿主分隔，建立了 Transwell 共培养模型。将肠上皮细胞与位于基底外侧室的人肥大细胞系 HMC-1.2 共培养，用脂多糖（LPS）刺激，然后用益生菌处理。在 HT29/HMC-1.2 共培养中，益生菌配方能够抵消 LPS 诱导的 HMC-1.2 中白细胞介素 6 的释放，并有效地维持 HT29/Caco-2/HMC-1.2 共培养中的上皮屏障完整性。这些结果表明该益生菌配方具有潜在的治疗效果。
