Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha 410012, China.
The Center for Heart Development, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Int J Mol Sci. 2023 Feb 20;24(4):4180. doi: 10.3390/ijms24044180.
Sleep-wake stability is imbalanced with natural aging, and microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and aging; however, the biological functions of miRNAs in regulating aging-related sleep-wake behavior remain unexplored. This study varied the expression pattern of dmiR-283 in and the result showed that the aging decline in sleep-wake behavior was caused by the accumulation of brain dmiR-283 expression, whereas the core clock genes and Notch signaling pathway might be suppressed, which regulate the aging process. In addition, to identify exercise intervention programs of that promote healthy aging, mir-283/+ and > mir-283 flies were driven to perform endurance exercise for a duration of 3 weeks starting at 10 and 30 days, respectively. The results showed that exercise starting in youth leads to an enhanced amplitude of sleep-wake rhythms, stable periods, increased activity frequency upon awakening, and the suppression of aging brain dmiR-283 expression in mir-283/+ middle-aged flies. Conversely, exercise performed when the brain dmiR-283 reached a certain accumulation level showed ineffective or negative effects. In conclusion, the accumulation of dmiR-283 expression in the brain induced an age-dependent decline in sleep-wake behavior. Endurance exercise commencing in youth counteracts the increase in dmiR-283 in the aging brain, which ameliorates the deterioration of sleep-wake behavior during aging.
睡眠-觉醒稳定性随自然衰老而失衡，微小 RNA（miRNAs）在细胞增殖、凋亡和衰老中发挥重要作用；然而，miRNAs 调节与衰老相关的睡眠-觉醒行为的生物学功能仍未被探索。本研究改变了 和中 dmiR-283 的表达模式，结果表明，睡眠-觉醒行为的衰老下降是由于大脑 dmiR-283 表达的积累引起的，而核心时钟基因 和 Notch 信号通路可能受到抑制，从而调节衰老过程。此外，为了确定促进健康衰老的 锻炼干预方案，将 mir-283/+ 和 >mir-283 苍蝇分别在 10 天和 30 天开始进行为期 3 周的耐力运动。结果表明，年轻时开始运动可增强睡眠-觉醒节律的振幅、稳定期、唤醒后活动频率增加，并抑制 mir-283/+ 中年苍蝇大脑中衰老的 dmiR-283 表达。相反，当大脑 dmiR-283 达到一定积累水平时进行运动则无效或产生负面影响。总之，大脑中 dmiR-283 表达的积累导致与年龄相关的睡眠-觉醒行为下降。年轻时开始的耐力运动可抵消衰老大脑中 dmiR-283 的增加，从而改善衰老过程中睡眠-觉醒行为的恶化。
