Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Faculty of Dentistry, The University of Otago, Dunedin 9054, New Zealand.
Int J Mol Sci. 2023 Feb 20;24(4):4247. doi: 10.3390/ijms24044247.
The objective of this study was to review the design methods that have been used to create peptides for use in caries management. Two independent researchers systematically reviewed many in vitro studies in which peptides were designed for use in caries management. They assessed the risk of bias in the included studies. This review identified 3592 publications, of which 62 were selected. Forty-seven studies reported 57 antimicrobial peptides. Among them, 31 studies (66%, 31/47) used the template-based design method; 9 studies (19%, 9/47) used the conjugation method; and 7 studies (15%, 7/47) used other methods, such as the synthetic combinatorial technology method, the de novo design method and cyclisation. Ten studies reported mineralising peptides. Seven of these (70%, 7/10) used the template-based design method, two (20%, 2/10) used the de novo design method, and one study (10%, 1/10) used the conjugation method. In addition, five studies developed their own peptides with antimicrobial and mineralising properties. These studies used the conjugation method. Our assessment for the risk of bias in the 62 reviewed studies showed that 44 publications (71%, 44/62) had a medium risk and that 3 publications had a low risk (5%, 3/62). The two most common methods for developing peptides for use in caries management that were used in these studies were the template-based design method and the conjugation method.
本研究旨在综述用于龋病管理的肽设计方法。两位独立研究者系统地回顾了许多体外研究，这些研究设计了用于龋病管理的肽。他们评估了纳入研究的偏倚风险。本综述共确定了 3592 篇文献，其中有 62 篇被选中。47 项研究报告了 57 种抗菌肽。其中，31 项研究（66%，31/47）采用基于模板的设计方法；9 项研究（19%，9/47）采用缀合方法；7 项研究（15%，7/47）采用其他方法，如合成组合技术方法、从头设计方法和环化。10 项研究报告了矿化肽。其中 7 项研究（70%，7/10）采用基于模板的设计方法，2 项研究（20%，2/10）采用从头设计方法，1 项研究（10%，1/10）采用缀合方法。此外，有 5 项研究开发了具有抗菌和矿化特性的自有肽。这些研究采用了缀合方法。我们对 62 篇综述研究的偏倚风险评估表明，44 篇出版物（71%，44/62）存在中度风险，3 篇出版物存在低度风险（5%，3/62）。这两种最常用于开发用于龋病管理的肽的方法是基于模板的设计方法和缀合方法。
