Chen Jine, Du Xin, Xu Xia, Zhang Sheng, Yao Lusong, He Xiuling, Wang Yongqiang
Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Proteomics and Metabolomics Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA.
Insects. 2023 Jan 28;14(2):134. doi: 10.3390/insects14020134.
Artificial parthenogenetic induction via thermal stimuli in silkworm is an important technique that has been used in sericultural production. However, the molecular mechanism underlying it remains largely unknown. We have created a fully parthenogenetic line (PL) with more than 85% occurrence and 80% hatching rate via hot water treatment and genetic selection, while the parent amphigenetic line (AL) has less than 30% pigmentation rate and less than 1% hatching rate when undergoing the same treatment. Here, isobaric tags for relative and absolute quantitation (iTRAQ)-based analysis were used to investigate the key proteins and pathways associated with silkworm parthenogenesis. We uncovered the unique proteomic features of unfertilized eggs in PL. In total, 274 increased abundance proteins and 211 decreased abundance proteins were identified relative to AL before thermal induction. Function analysis displayed an increased level of translation and metabolism in PL. After thermal induction, 97 increased abundance proteins and 187 decreased abundance proteins were identified. An increase in stress response-related proteins and decrease in energy metabolism suggested that PL has a more effective response to buffer the thermal stress than AL. Cell cycle-related proteins, including histones, and spindle-related proteins were decreased in PL, indicating an important role of this decrease in the process of ameiotic parthenogenesis.
通过热刺激在家蚕中诱导人工孤雌生殖是一项已应用于养蚕生产的重要技术。然而，其潜在的分子机制仍 largely 未知。我们通过热水处理和遗传选择创建了一个完全孤雌生殖品系（PL），其发生率超过 85%，孵化率达 80%，而亲本两性生殖品系（AL）在接受相同处理时，色素沉着率低于 30%，孵化率低于 1%。在此，基于相对和绝对定量的等压标签（iTRAQ）分析被用于研究与家蚕孤雌生殖相关的关键蛋白质和途径。我们揭示了 PL 中未受精卵独特的蛋白质组学特征。相对于热诱导前的 AL，总共鉴定出 274 种丰度增加的蛋白质和 211 种丰度降低的蛋白质。功能分析显示 PL 中翻译和代谢水平增加。热诱导后，鉴定出 97 种丰度增加的蛋白质和 187 种丰度降低的蛋白质。应激反应相关蛋白质增加而能量代谢降低表明 PL 比 AL 对缓冲热应激具有更有效的反应。PL 中包括组蛋白在内的细胞周期相关蛋白质和纺锤体相关蛋白质减少，表明这种减少在减数分裂孤雌生殖过程中起重要作用。
