Reduta Teresa, Bacharewicz-Szczerbicka Joanna, Stasiak-Barmuta Anna, Kaminski Tomasz W, Flisiak Iwona
Department of Dermatology and Venereology, Medical University of Bialystok, Zurawia 14 St., 15-540 Bialystok, Poland.
Department of Clinical Immunology, Medical University of Bialystok, Waszyngtona 17 St., 15-274 Bialystok, Poland.
J Clin Med. 2023 Feb 9;12(4):1397. doi: 10.3390/jcm12041397.
Studies have shown that osteopontin (OPN) and regulatory T cells play a role in allergic contact dermatitis (ACD), but the mechanisms responsible for their function are poorly understood. The study aimed to determine CD4 T lymphocytes producing intracellular osteopontin (iOPN T cells) and assess the selected T lymphocyte subsets including regulatory T cells in the blood of patients with ACD. Twenty-six patients with a disseminated form of allergic contact dermatitis and 21 healthy controls were enrolled in the study. Blood samples were taken twice: in the acute phase of the disease and during remission. The samples were analyzed by the flow cytometry method. Patients with acute ACD showed significantly higher percentage of iOPN T cells compared with healthy controls which persisted during remission. An increase in the percentage of CD4CD25 and a reduced percentage of regulatory T lymphocytes (CD4CD25highCD127low) were also found in the patients with acute stage of ACD. The percentage of CD4CD25 T lymphocytes showed a positive correlation with the EASI index. The increase in the iOPN T cells can indicate their participation in acute ACD. The decreased percentage of regulatory T lymphocytes in the acute stage of ACD may be related to the transformation of Tregs into CD4CD25 T cells. It may also indicate their increased recruitment to the skin. The positive correlation between the percentage of CD4CD25 lymphocytes and the EASI index may be indirect evidence for the importance of activated lymphocytes-CD4CD25 in addition to CD8 lymphocytes as effector cells in ACD.
研究表明，骨桥蛋白（OPN）和调节性T细胞在过敏性接触性皮炎（ACD）中发挥作用，但其功能所涉及的机制尚不清楚。该研究旨在确定产生细胞内骨桥蛋白的CD4 T淋巴细胞（iOPN T细胞），并评估ACD患者血液中包括调节性T细胞在内的特定T淋巴细胞亚群。26例播散型过敏性接触性皮炎患者和21名健康对照者纳入该研究。采集两次血样：疾病急性期和缓解期。采用流式细胞术对样本进行分析。急性ACD患者的iOPN T细胞百分比显著高于健康对照者，且在缓解期持续存在。在急性ACD期患者中还发现CD4CD25百分比增加，调节性T淋巴细胞（CD4CD25highCD127low）百分比降低。CD4CD25 T淋巴细胞百分比与EASI指数呈正相关。iOPN T细胞增加表明它们参与急性ACD。ACD急性期调节性T淋巴细胞百分比降低可能与调节性T细胞向CD4CD25 T细胞转化有关。这也可能表明它们向皮肤的募集增加。CD4CD25淋巴细胞百分比与EASI指数之间的正相关可能是除CD8淋巴细胞作为ACD效应细胞外，活化淋巴细胞CD4CD25重要性的间接证据。
