Semenycheva Ludmila, Chasova Victoria, Fukina Diana, Koryagin Andrey, Belousov Artem, Valetova Natalia, Suleimanov Evgeny
The Research Institute for Chemistry, Lobachevsky State University of Nizhny Novgorod, pr. Gagarina 23, Nizhny Novgorod 603950, Russia.
Life (Basel). 2023 Jan 28;13(2):352. doi: 10.3390/life13020352.
Graft copolymerization of methyl methacrylate onto cod collagen was carried out under visible light irradiation (λ = 400-700 nm) at 20-25 °C using the RbTeWO, CsTeMoO, and RbNbTeO complex oxides with β-pyrochlore structure as photocatalysts. The as-prepared materials were characterized by X-ray diffraction, scanning electron microscopy, and UV-Vis diffuse reflectance spectroscopy. It was also found that RbNbTeO with β-pyrochlore structure was not able to photocatalyze the reaction. Enzymatic hydrolysis of the obtained graft copolymers proceeds with the formation of peptides with a molecular weight (MW) of about 20 and 10 kDa. In contrast to collagen, which decomposes predominantly to peptides with MW of about 10 kDa, the ratio of fractions with MW of about 10 kDa and 20 kDa differs much less, their changes are symbatic, and the content of polymers with MW of more than 20 kDa is about 70% after 1 h in the case of graft copolymers. The data obtained indicate that synthetic fragments grafted to the collagen macromolecule do not prevent the hydrolysis of the peptide bonds but change the rate of polymer degradation. This is important for creating network matrix scaffolds based on graft copolymers by cross-linking peptides, which are products of enzymatic hydrolysis.
以具有β-烧绿石结构的RbTeWO、CsTeMoO和RbNbTeO复合氧化物为光催化剂，在20 - 25°C可见光照射（λ = 400 - 700 nm）下，进行甲基丙烯酸甲酯接枝共聚到鳕鱼胶原蛋白上的反应。通过X射线衍射、扫描电子显微镜和紫外 - 可见漫反射光谱对所制备的材料进行表征。还发现具有β-烧绿石结构的RbNbTeO不能光催化该反应。所得接枝共聚物的酶促水解过程中会形成分子量（MW）约为20 kDa和10 kDa的肽。与主要分解为分子量约为10 kDa的肽的胶原蛋白不同，分子量约为10 kDa和20 kDa的组分比例差异小得多，它们的变化是同步的，对于接枝共聚物而言，1小时后分子量大于20 kDa的聚合物含量约为70%。所获得的数据表明，接枝到胶原蛋白大分子上的合成片段不会阻止肽键的水解，但会改变聚合物的降解速率。这对于通过交联酶促水解产物肽来制备基于接枝共聚物的网络基质支架很重要。
