Department of Periodontics, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College, Saveetha University, Chennai 600077, India.
Department of Medical Education, Nawaz Sharif Medical College, Gujrat 50700, Pakistan.
Medicina (Kaunas). 2023 Feb 6;59(2):302. doi: 10.3390/medicina59020302.
: Periodontitis is a chronic multifactorial inflammatory infectious disease marked by continuous degradation of teeth and surrounding parts. One of the most important periodontal pathogens is , and with its interpain A proteinase, it leads to an increase in lethal infection. : The current study was designed to create a multi-epitope vaccine using an immunoinformatics method that targets the interpain A of . For the development of vaccines, peptides InpA were found appropriate. To create a multi-epitope vaccination design, interpain A, B, and T-cell epitopes were found and assessed depending on the essential variables. The vaccine construct was evaluated based on its stability, antigenicity, and allergenicity. The vaccine construct reached a more significant population and was able to bind to both the binding epitopes of major histocompatibility complex (MHC)-I and MHC-II. Through the C3 receptor complex route, InpA promotes an immunological subunit. Utilizing InpA-C3 and vaccination epitopes as the receptor and ligand, the molecular docking and dynamics were performed using the ClusPro 2.0 server. : The developed vaccine had shown good antigenicity, solubility, and stability. Molecular docking indicated the vaccine's 3D structure interacts strongly with the complement C3. The current study describes the design for vaccine, and steady interaction with the C3 immunological receptor to induce a good memory and an adaptive immune response against Interpain A of .
牙周炎是一种慢性多因素炎症感染性疾病，其特征是牙齿及周围组织的持续破坏。最重要的牙周病原体之一是 ，其 interpain A 蛋白酶导致致死性感染增加。本研究旨在采用免疫信息学方法，针对 interpain A 设计一种多表位疫苗。为了开发疫苗，发现 InpA 肽是合适的。为了创建多表位疫苗设计，发现了 interpain A、B 和 T 细胞表位，并根据必要变量进行了评估。根据其稳定性、抗原性和过敏性对疫苗结构进行了评估。疫苗结构达到了更高的人群覆盖率，并且能够与主要组织相容性复合体 (MHC)-I 和 MHC-II 的结合表位结合。通过 C3 受体复合物途径，InpA 促进免疫亚单位。利用 InpA-C3 和疫苗表位作为受体和配体，使用 ClusPro 2.0 服务器进行了分子对接和动力学模拟。所开发的疫苗具有良好的抗原性、可溶性和稳定性。分子对接表明，疫苗的 3D 结构与补体 C3 强烈相互作用。本研究描述了针对 interpain A 的疫苗设计，以及与 C3 免疫受体的稳定相互作用，以诱导针对 Interpain A 的良好记忆和适应性免疫反应。
