Coimbra Pedro Paulo Saldanha, Silva-E-Silva Anna Carolina Alves Gomes da, Antonio Ananda da Silva, Pereira Henrique Marcelo Gualberto, Veiga-Junior Valdir Florêncio da, Felzenszwalb Israel, Araujo-Lima Carlos Fernando, Teodoro Anderson Junger
Food and Nutrition Graduate Program, Federal University of Rio de Janeiro State, Rio de Janeiro 21941-901, Brazil.
Laboratory of Environmental Mutagenicity, Department of Biophysics and Biometry, Rio de Janeiro State University, Rio de Janeiro 20550-013, Brazil.
Metabolites. 2023 Feb 14;13(2):277. doi: 10.3390/metabo13020277.
In this study, a beetroot peel flour was made, and its in vitro antioxidant activity was determined in aqueous (BPFw) and ethanolic (BPFe) extracts. The influence of BPFw on breast cancer cell viability was also determined. A targeted betalain profile was obtained using high-resolution Q-Extractive Plus Orbitrap mass spectrometry (Obrtitrap-HRMS) alongside untargeted chemical profiling of BPFw using Ultra-High-Performance Liquid Chromatography with High-Resolution Mass Spectrometry (UHPLC-HRMS). BPFw and BPFe presented satisfactory antioxidant activities, with emphasis on the total phenolic compounds and ORAC results for BPFw (301.64 ± 0.20 mg GAE/100 g and 3032.78 ± 55.00 µmol T/100 g, respectively). The MCF-7 and MDA-MB-231 breast cancer cells presented reductions in viability when treated with BPFw, showing dose-dependent behavior, with MDA-MB-231 also showing time-dependent behavior. The chemical profiling of BPFw led to the identification of 9 betalains and 59 other compounds distributed amongst 28 chemical classes, with flavonoids and their derivates and coumarins being the most abundant. Three forms of betalain generated via thermal degradation were identified. However, regardless of thermal processing, the BPF still presented satisfactory antioxidant and anticancer activities, possibly due to synergism with other identified molecules with reported anticancer activities via different metabolic pathways.
在本研究中，制备了甜菜根皮粉，并测定了其水提取物（BPFw）和乙醇提取物（BPFe）的体外抗氧化活性。还测定了BPFw对乳腺癌细胞活力的影响。使用高分辨率Q-提取加轨道阱质谱（Obrtitrap-HRMS）获得了靶向的甜菜色素谱，并使用超高效液相色谱-高分辨率质谱（UHPLC-HRMS）对BPFw进行了非靶向化学分析。BPFw和BPFe表现出令人满意的抗氧化活性，尤其是BPFw的总酚类化合物和ORAC结果（分别为301.64±0.20 mg GAE/100 g和3032.78±55.00 µmol T/100 g）。用BPFw处理时，MCF-7和MDA-MB-231乳腺癌细胞的活力降低，呈现剂量依赖性，MDA-MB-231还呈现时间依赖性。BPFw的化学分析鉴定出9种甜菜色素和59种其他化合物，分布在28个化学类别中，其中黄酮类及其衍生物和香豆素最为丰富。鉴定出了三种通过热降解产生的甜菜色素形式。然而，无论热处理如何，BPF仍表现出令人满意的抗氧化和抗癌活性，这可能是由于与其他已鉴定的具有抗癌活性的分子通过不同代谢途径产生协同作用。
