不同方法测定含甜菜红素产品中植物化学物质和抗氧化活性的比较评价——验证甜菜根样品。
Comparative evaluation of different methods for determining phytochemicals and antioxidant activity in products containing betalains - Verification of beetroot samples.
机构信息
Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 St., 80-233 Gdańsk, Poland; BioTechMed Center, Gdańsk University of Technology, Narutowicza 11/12 St., 80-233 Gdańsk, Poland.
Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, Narutowicza 11/12 St., 80-233 Gdańsk, Poland.
出版信息
Food Chem. 2021 Nov 15;362:130132. doi: 10.1016/j.foodchem.2021.130132. Epub 2021 May 15.
This study presents methods that can be used to assess the health quality of products containing betalains. The paper compares and verifies data on the phytochemical composition of three different pigmented beetroot cultivars using spectrophotometric, HPLC-DAD, HPTLC and LC-Q-Orbitrap-HRMS techniques. Additionally, we compared the total antioxidant activity in both the cell-free and cellular systems. Betalain contribution to antioxidant activity was also determined using post-column derivatization and it was found that in the case of red beetroot it is about 50%. Photometric measurements are recommended for a simple and inexpensive analysis of the total betacyanin and betaxanthin content. Liquid chromatography techniques produced more precise information on phytochemical composition in the tested samples. The combination of liquid chromatography with high-resolution mass spectrometry produced the largest amount of quantitative and qualitative data; in beetroot samples sixty-four phytochemicals have been identified therefore, this approach is recommended for more detailed metabolomics studies.
本研究提出了可用于评估含甜菜红素产品健康质量的方法。本文使用分光光度法、HPLC-DAD、HPTLC 和 LC-Q-Orbitrap-HRMS 技术，比较和验证了三种不同花色苷根甜菜品种的植物化学成分数据。此外，我们还比较了细胞外和细胞内系统的总抗氧化活性。使用柱后衍生化法确定了甜菜红素对抗氧化活性的贡献，结果发现，在红甜菜根中，其贡献约为 50%。分光光度法测量建议用于简单且廉价的总甜菜红素和甜菜黄素含量分析。液相色谱技术为测试样品的植物化学成分提供了更精确的信息。液相色谱与高分辨率质谱相结合产生了最多的定量和定性数据；在甜菜根样品中鉴定出了 64 种植物化学物质，因此，建议采用这种方法进行更详细的代谢组学研究。
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