Bezek Katja, Avberšek Jana, Zorman Rojs Olga, Barlič-Maganja Darja
Faculty of Health Sciences, University of Primorska, 6310 Izola, Slovenia.
Veterinary Faculty, Institute of Microbiology and Parasitology, University of Ljubljana, 1000 Ljubljana, Slovenia.
Microorganisms. 2023 Jan 23;11(2):301. doi: 10.3390/microorganisms11020301.
subsp. serovar Infantis is the most prevalent serovar in broilers and broiler meat in the European Union. The aim of our study was to test the biofilm formation and antimicrobial effect of disinfectants on genetically characterized Infantis isolates from poultry, food, and humans. For the biofilm formation under various temperature conditions (8 °C, 20 °C, and 28 °C) and incubation times (72 h and 168 h), the crystal violet staining method was used. The evaluation of the in vitro antimicrobial effect of Ecocid S, ethanol, and hydrogen peroxide was determined using the broth microdilution method. The antibiofilm effect of subinhibitory concentration (1/8 MIC) of disinfectants was then tested on Infantis 323/19 strain that had the highest biofilm formation potential. Our results showed that the biofilm formation was strain-specific; however, it was higher at 20 °C and prolonged incubation time. Moreover, strains carrying a pESI plasmid showed higher biofilm formation potential. The antibiofilm potential of disinfectants on Infantis 323/19 strain at 20 °C was effective after a shorter incubation time. As shown in our study, more effective precautionary measures should be implemented to ensure biofilm prevention and removal in order to control the Infantis occurrence.
亚种 血清型婴儿亚种是欧盟肉鸡和鸡肉中最常见的血清型。我们研究的目的是测试消毒剂对来自家禽、食品和人类的基因特征明确的婴儿亚种分离株的生物膜形成和抗菌效果。对于在各种温度条件（8℃、20℃和28℃）和培养时间（72小时和168小时）下的生物膜形成，使用了结晶紫染色法。使用肉汤微量稀释法测定了Ecocid S、乙醇和过氧化氢的体外抗菌效果。然后在具有最高生物膜形成潜力的婴儿亚种323/19菌株上测试了消毒剂亚抑制浓度（1/8 MIC）的抗生物膜效果。我们的结果表明，生物膜形成具有菌株特异性；然而，在20℃和延长培养时间时生物膜形成更高。此外，携带pESI质粒的菌株显示出更高的生物膜形成潜力。消毒剂对20℃下的婴儿亚种323/19菌株的抗生物膜潜力在较短培养时间后有效。如我们的研究所示，应实施更有效的预防措施以确保生物膜的预防和清除，从而控制婴儿亚种的出现。
