Yin Xuejiao, Duan Chunhui, Ji Shoukun, Tian Peizhi, Ju Sisi, Yan Hui, Zhang Yingjie, Liu Yueqin
College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.
Microorganisms. 2023 Jan 30;11(2):348. doi: 10.3390/microorganisms11020348.
Colonization of gastrointestinal microbiota in mammals during early life is vital to host health. The objective of this study was to investigate whether lambs with high and low ADG have a different rumen and rectum microbial community. Thus, we investigated potential relationships between rumen and rectum microbiota and average daily gain (ADG) in weaned lambs. Sixteen lambs with similar body weights (7.63 ± 1.18 kg) were selected at 30 days of age. At 60 days of age, lambs were weaned, and ADG was calculated from 60 to 90 days. Then, two groups were generated: higher ADG (HG, 134.17 ± 13.48 g/day) and lower ADG (LG, 47.50 ± 19.51 g/day). Microbiota was evaluated at 30, 60, and 90 days of age. The final live weight and ADG at 90 days of age was higher ( < 0.05) in the HG group compared to the LG group. The maturity of bacterial and fungal communities was increased ( < 0.05) in the HG group for the 30 days vs. 90 days comparison and 60 days vs. 90 days comparison. Linear discriminant analysis effect size (LEfSe) analysis revealed a total of 18 bacterial biomarkers that are ADG-specific in the rumen and 35 bacterial biomarkers in the rectum. Meanwhile, 15 fungal biomarkers were found in the rumen and 8 biomarkers were found in the rectum. Our findings indicated that ADG is related to the rumen and rectum microbiota in lambs.
哺乳动物早期生命阶段胃肠道微生物群的定殖对宿主健康至关重要。本研究的目的是调查平均日增重高低不同的羔羊瘤胃和直肠微生物群落是否存在差异。因此，我们研究了断奶羔羊瘤胃和直肠微生物群与平均日增重（ADG）之间的潜在关系。在30日龄时选择了16只体重相近（7.63±1.18千克）的羔羊。在60日龄时给羔羊断奶，并计算60至90日龄期间的ADG。然后，分为两组：较高ADG组（HG，134.17±13.48克/天）和较低ADG组（LG，47.50±19.51克/天）。在30、60和90日龄时评估微生物群。与LG组相比，HG组90日龄时的最终活重和ADG更高（P<0.05）。在HG组中，30天与90天比较以及60天与90天比较时，细菌和真菌群落的成熟度增加（P<0.05）。线性判别分析效应大小（LEfSe）分析显示，瘤胃中共有18种细菌生物标志物是ADG特异性的，直肠中有35种细菌生物标志物。同时，在瘤胃中发现了15种真菌生物标志物，在直肠中发现了8种生物标志物。我们的研究结果表明，ADG与羔羊的瘤胃和直肠微生物群有关。
