Department of Nursing, College of Applied Medical Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Department of Public Health, College of Applied Medical Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Molecules. 2023 Feb 7;28(4):1578. doi: 10.3390/molecules28041578.
is rich in bioactive compounds such as beta-carotene, which have high nutritional values and antimicrobial applications. Several studies have confirmed that bioactive-compound-based herbal medicines extracted from the leaves, seeds, fruits and shoots of are vital to cure many diseases and infections, and for the healing of wounds. The β-carotene is a naturally occurring bioactive compound encoded by zeta-carotene desaturase (ZDS) and phytoene synthase (PSY) genes. In the current study, computational analyses were performed to identify and characterize ZDS and PSY genes retrieved from (as reference) and these were compared with the corresponding genes in , , and . The BLAST results revealed that all the plant species considered in this study encode β-carotene genes with 80-100% similarity. The Pfam analysis on β-carotene genes of all the investigated plants confirmed that they belong to the same protein family and domain. Similarly, phylogenetic analysis revealed that β-carotene genes of belong to the same ancestral class. Using the ZDS and PSY genes of as a reference, we conducted qRT-PCR analysis on RNA extracted from the leaves of , , and . It was noted that the most significant gene expression occurred in the leaves of the studied medicinal plants. We concluded that not only are the leaves of an effective source of bioactive compounds including beta carotene, but also the leaves of , and can be employed as antibiotics and antioxidants against bacterial or microbial infections.
是一种富含生物活性化合物的植物，如β-胡萝卜素，具有很高的营养价值和抗菌应用。多项研究证实，从叶子、种子、果实和嫩枝中提取的基于生物活性化合物的草药对治疗许多疾病和感染以及伤口愈合至关重要。β-胡萝卜素是一种由 ζ-胡萝卜素去饱和酶（ZDS）和八氢番茄红素合酶（PSY）基因编码的天然存在的生物活性化合物。在目前的研究中，进行了计算分析，以鉴定和表征从 （作为参考）中检索到的 ZDS 和 PSY 基因，并将其与 、 、 和 中的相应基因进行比较。BLAST 结果表明，本研究中考虑的所有植物物种都编码具有 80-100%相似性的β-胡萝卜素基因。对所有研究植物的β-胡萝卜素基因的 Pfam 分析证实，它们属于同一蛋白家族和结构域。同样，系统发育分析表明， 的β-胡萝卜素基因属于同一祖先类群。使用 中的 ZDS 和 PSY 基因作为参考，我们对 、 、 和 叶片中提取的 RNA 进行了 qRT-PCR 分析。结果表明，基因表达最显著的是研究药用植物的叶片。我们得出结论，不仅 的叶子是包括β-胡萝卜素在内的生物活性化合物的有效来源，而且 的叶子也可以用作抗生素和抗氧化剂，对抗细菌或微生物感染。
