Department of Pharmaceutical Sciences, School of Pharmacy, Sefako Makgatho Health Sciences University, Molotlegi Street, Ga-Rankuwa, Pretoria 0204, South Africa.
Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa.
Molecules. 2023 Jan 16;28(2):899. doi: 10.3390/molecules28020899.
, native to India, grows in tropical and subtropical regions around the world and has valuable pharmacological properties such as anti-asthmatic, anti-diabetic, anti-inflammatory, anti-infertility, anti-cancer, anti-microbial, antioxidant, and many more. The purpose of this study was to assess the free radical scavenging ability of two extracts and two pure compounds of Lam (hexane, ethanol, compound E3, and compound Ra) against reactive oxygen species, as well as their reducing power and antimicrobial activities. Bioautography antioxidant assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (HO) free radical scavenging, and iron (iii) (Fe to Fe) chloride reducing power assays were used to assess the extracts' qualitative and quantitative free radical scavenging activities. Furthermore, the extract and the compounds were tested against both Gram-positive and Gram-negative bacterial strains suspended in Mueller-Hinton Broth. The extracts and pure compounds showed noteworthy antioxidant potential, with positive compound bands in the Rf range of 0.05-0.89. DPPH), HO, and Fe to Fe reduction assays revealed that ethanol extract has a high antioxidant potential, followed by compound E3, compound Ra, and finally hexane extract. Using regression analysis, the half maximal inhibitory concentration (IC) values for test and control samples were calculated. Compound Ra and ethanol exhibited high antioxidant activity at concentrations as low as ≈0.28 mg/mL in comparison with n-hexane extract, compound E3, ascorbic acid, and butylated hydroxytoluene standards. The radical scavenging activity of almost all plant extracts against DPPH was observed at 0.28 mg/mL; however, the highest activity was observed at the same concentration for ascorbic acid and butylated hydroxytoluene (BHT) with a low IC value of 0.08 mg/mL and compound Ra and ethanol with a low IC of 0.4 mg/mL, respectively. The extracts and pure compounds of have little to no antibacterial potential. extracts contain antioxidant agents efficient to alleviate degenerative conditions such as cancer and cardiovascular disease but have little activity against infectious diseases.
，原产于印度，生长在世界各地的热带和亚热带地区，具有宝贵的药理学特性，如抗哮喘、抗糖尿病、抗炎、抗不孕、抗癌、抗微生物、抗氧化等。本研究的目的是评估两种提取物和两种纯化合物（六烷、乙醇、化合物 E3 和化合物 Ra）对活性氧的自由基清除能力，以及它们的还原能力和抗菌活性。生物自显影抗氧化测定法、2,2-二苯基-1-苦基肼（DPPH）、过氧化氢（HO）自由基清除测定法和铁（iii）（Fe 至 Fe）氯化物还原能力测定法用于评估提取物的定性和定量自由基清除活性。此外，提取物和化合物都在 Mueller-Hinton 肉汤中针对革兰氏阳性和革兰氏阴性细菌菌株进行了测试。提取物和纯化合物表现出显著的抗氧化潜力，在 Rf 范围为 0.05-0.89 处有阳性化合物带。DPPH）、HO 和 Fe 至 Fe 还原测定法表明，乙醇提取物具有较高的抗氧化潜力，其次是化合物 E3、化合物 Ra，最后是正己烷提取物。使用回归分析，计算了测试和对照样品的半最大抑制浓度（IC）值。与正己烷提取物、化合物 E3、抗坏血酸和丁基化羟基甲苯标准品相比，化合物 Ra 和乙醇在低至约 0.28mg/ml 的浓度下表现出高抗氧化活性。几乎所有植物提取物对 DPPH 的自由基清除活性在 0.28mg/ml 时观察到；然而，在相同浓度下，抗坏血酸和丁基化羟基甲苯（BHT）的活性最高，IC 值低至 0.08mg/ml，化合物 Ra 和乙醇的 IC 值低至 0.4mg/ml。和乙醇的提取物和纯化合物几乎没有抗菌潜力。提取物含有有效缓解癌症和心血管疾病等退行性疾病的抗氧化剂，但对传染病几乎没有活性。
