Kessler J P, Moyse E, Kitabgi P, Vincent J P, Beaudet A
Montreal Neurological Institute, McGill University, Quebec, Canada.
Neuroscience. 1987 Oct;23(1):189-98. doi: 10.1016/0306-4522(87)90282-x.
Specific high-affinity neurotensin binding sites were labeled in sections of the rat caudal brainstem using a monoiodinated ligand, and their distribution was examined by light microscopic radioautography after fixation with glutaraldehyde. In the medulla, labeled binding sites were mainly concentrated within the dorsal motor nucleus of the vagus, the nucleus of the solitary tract, the external cuneate nucleus, the lateral reticular nucleus, the medial vestibular nucleus, the retrofacial nucleus, the linearis nucleus, the paragigantocellular nucleus and the nucleus raphe pallidus. Within the pons, neurotensin binding sites were detected in the reticulotegmental nucleus, the pontine nuclei, the dorsal tegmental nucleus, the laterodorsal and pedunculopontine tegmental nuclei and the nuclei raphe dorsalis and medianus. Most nuclei found here to contain high densities of neurotensin binding sites have been shown to stain intensely for acetylcholinesterase, suggesting a possible association between this enzyme and neurotensin receptors.
使用单碘化配体对大鼠尾侧脑干切片中的特异性高亲和力神经降压素结合位点进行标记，并在戊二醛固定后通过光学显微镜放射自显影检查其分布。在延髓中，标记的结合位点主要集中在迷走神经背运动核、孤束核、楔外核、外侧网状核、内侧前庭核、面神经后核、直线核、巨细胞旁核和中缝苍白核内。在脑桥内，在网状被盖核、脑桥核、背侧被盖核、外侧背侧和脚桥被盖核以及中缝背核和中缝正中核中检测到神经降压素结合位点。此处发现的大多数含有高密度神经降压素结合位点的核已被证明对乙酰胆碱酯酶有强烈染色，表明该酶与神经降压素受体之间可能存在关联。
