College of Public Health, Zhengzhou University, Zhengzhou 540001, China.
Institute of Chronic Disease Risks Assessment, School of Nursing and Health, Henan University, Kaifeng 475004, China.
Nutrients. 2023 Feb 10;15(4):907. doi: 10.3390/nu15040907.
Naringin (Nar) is a dihydroflavonoid compound, widely found in citrus fruit and used in Chinese herbal medicine. As a phytochemical, it acts as a dietary supplement that can delay aging and prevent aging-related disease, such as obesity and diabetes. However, its exact mechanism remains unclear. In this study, the high-glucose-induced (HGI) model was used to evaluate the anti-aging and anti-obesity effects of Nar. The mean lifespan and fast movement span of HGI worms were extended roughly 24% and 11%, respectively, by Nar treatment. Oil red O staining revealed a significant reduction in fat accumulation and dFP::LGG-labeled worms showed the promotion of autophagy. Additionally, whole transcriptome sequencing and gene set variation analysis suggested that Nar upregulated the lipid biosynthesis and metabolism pathways, as well as the TGF-β, Wnt and longevity signaling pathways. Protein-protein interaction (PPI) network analysis identified hub genes in these pathways for further analysis. Mutant worms and RNA interference were used to study mechanisms; the suppression of , , , , and disabled the fat-lowering, lifespan-prolonging, and health-promoting properties of Nar. Collectively, our findings indicate that Nar plays an important role in alleviating HGI-aging and anti-obesity effects by reducing fat accumulation and promoting autophagy.
柚皮苷（Nar）是一种二氢黄酮类化合物，广泛存在于柑橘类水果中，用于中药。作为一种植物化学物质，它作为膳食补充剂，可以延缓衰老和预防与衰老相关的疾病，如肥胖和糖尿病。然而，其确切的机制尚不清楚。在这项研究中，使用高葡萄糖诱导（HGI）模型来评估 Nar 的抗衰老和抗肥胖作用。Nar 处理可使 HGI 蠕虫的平均寿命和快速运动跨度分别延长约 24%和 11%。油红 O 染色显示脂肪积累明显减少，dFP::LGG 标记的蠕虫显示自噬促进。此外，全转录组测序和基因集变异分析表明，Nar 上调了脂质生物合成和代谢途径，以及 TGF-β、Wnt 和长寿信号通路。蛋白质-蛋白质相互作用（PPI）网络分析确定了这些途径中的枢纽基因进行进一步分析。突变蠕虫和 RNA 干扰用于研究机制；抑制 、 、 、 和 使 Nar 的降脂、延长寿命和促进健康的特性丧失。总之，我们的研究结果表明，Nar 通过减少脂肪积累和促进自噬，在缓解 HGI 衰老和抗肥胖作用方面发挥了重要作用。
