Department of Bio-Health Convergence Major, Duksung Women's University, Seoul 01369, Republic of Korea.
Department of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
Nutrients. 2023 Feb 19;15(4):1038. doi: 10.3390/nu15041038.
This study evaluates the immune-enhancing effects of on cyclophosphamide (CP)-induced immunosuppression in BALB/c mice. In vitro, the expressions of pro-inflammatory cytokines and MAPK signaling molecules in Raw264.7 cells were analyzed by ELISA and Western blot analysis. Moreover, cell proliferation, surface receptor expression, and cytotoxicity of NK-92 cells were examined by Cell Counting Kit-8, CytoTox96 assay, and flow cytometry, respectively. To investigate the immune-enhancing effects of selected strains in vivo, these strains were orally administered to BALB/c mice for 2 weeks, and CP was intraperitoneally injected. Then, liver, spleen, and whole blood were isolated from each animal. Administration of single strains or their mixture sustained the spleen weight, the counts of white blood cells compared to non-fed group. Splenocyte proliferation and NK cytotoxicity were significantly increased in all -fed groups. The frequency of B220+ cells was also significantly enhanced in splenocytes isolated from groups. In addition, the production of cytokines (TNF-α, IFN-γ) and antibodies was recovered in splenocyte supernatants isolated from groups. In conclusion, could be a suitable functional food additive for immune-enhancing effect.
本研究评估了[成分名称]对环磷酰胺（CP）诱导的 BALB/c 小鼠免疫抑制的免疫增强作用。在体外，通过 ELISA 和 Western blot 分析分析 Raw264.7 细胞中促炎细胞因子和 MAPK 信号分子的表达。此外，通过细胞计数试剂盒-8（CCK-8）、细胞毒性 96 检测试剂盒（Cytotox96）和流式细胞术分别检测 NK-92 细胞的增殖、表面受体表达和细胞毒性。为了研究选定[成分名称]菌株在体内的免疫增强作用，将这些菌株口服给予 BALB/c 小鼠 2 周，并腹腔注射 CP。然后从每个动物中分离肝脏、脾脏和全血。与未喂养组相比，单一[成分名称]菌株或其混合物的给药维持了脾脏重量和白细胞计数。所有[成分名称]喂养组的脾细胞增殖和 NK 细胞毒性均显著增加。分离自[成分名称]组的脾细胞中 B220+细胞的频率也显著增强。此外，从[成分名称]组分离的脾细胞上清液中细胞因子（TNF-α、IFN-γ）和抗体的产生得到恢复。总之，[成分名称]可以作为一种具有免疫增强作用的功能性食品添加剂。
