College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
Duksung Innovative Drug Center, Duksung Women's University, Seoul 01369, Republic of Korea.
Nutrients. 2024 Sep 18;16(18):3158. doi: 10.3390/nu16183158.
(), a species of unicellular algae, can accumulate large amounts of β-1,3-glucan paramylon, a polysaccharide, in its cytoplasm and has recently attracted interest as a bioproduct due to its various health benefits. In this study, the immune-enhancing effect of powder (EP) was investigated in vitro and in vivo.
In vitro, the production of NO and cytokines and the mechanism of the signaling pathway of β-1,3-glucan were identified in RAW264.7 cells. In vivo, cyclophosphamide-induced (CP-induced) immunosuppressed C57BL/6 female mice were orally administered with three different concentrations (100, 300, and 600 mg/kg) of EP daily. After 14 days, the organs and whole blood were collected from each animal for further study.
The weight loss of CP-treated mice was reversed by treatment with EP to levels comparable to those of control mice. In addition, the frequencies of NK1.1, CD3, CD4, CD8, and B220 in immune cells isolated from the spleen were increased by EP treatment compared with water or RG. The secretion of TNF-α, IFN-γ, and IL-12 from splenocytes was also increased by EP treatment, as was the level of IgM in the serum of the mice. Finally, EP treatment specifically upregulated the expression of dectin-1 in the liver of CP-treated mice.
could be a good candidate for a natural immune stimulator in the innate and adaptive response by secreting TNF-α, IFN-γ, and IL-12 through stimulating dectin-1 expression on the surface of immune cells.
（），一种单细胞藻类，可以在细胞质中积累大量的β-1,3-葡聚糖 paramylon，一种多糖，由于其各种健康益处，最近作为生物产品引起了关注。在这项研究中，研究了 粉末（EP）的体外和体内免疫增强作用。
在体外，通过 RAW264.7 细胞鉴定了 NO 和细胞因子的产生以及 β-1,3-葡聚糖信号通路的机制。在体内，用环磷酰胺（CP）诱导免疫抑制的 C57BL/6 雌性小鼠每天口服三种不同浓度（100、300 和 600mg/kg）的 EP。14 天后，从每只动物收集器官和全血进行进一步研究。
EP 处理逆转了 CP 处理小鼠的体重减轻，使其恢复到与对照小鼠相当的水平。此外，与水或 RG 相比，EP 处理增加了从脾中分离的免疫细胞中 NK1.1、CD3、CD4、CD8 和 B220 的频率。EP 处理还增加了脾细胞中 TNF-α、IFN-γ 和 IL-12 的分泌，以及小鼠血清中 IgM 的水平。最后，EP 处理特异性地上调了 CP 处理小鼠肝脏中 dectin-1 的表达。
通过刺激免疫细胞表面上的 dectin-1 表达，可能是通过分泌 TNF-α、IFN-γ 和 IL-12 来引发先天和适应性反应的天然免疫刺激剂的良好候选物。
