Randag Anna C, de Kroon Lieke, Otten Henny, Arias Claro-Handgraaf Cindy, Schimmer Barbara, Kortbeek Titia, van Rooij Jeroen, Stelma Foekje F
Rotterdam Eye Hospital, Schiedamse Vest 180, 3011 BH Rotterdam, The Netherlands.
Rotterdam Ophthalmic Institute, Schiedamse Vest 160, 3011 BH Rotterdam, The Netherlands.
Pathogens. 2023 Jan 29;12(2):214. doi: 10.3390/pathogens12020214.
keratitis is almost universally associated with contact lens (CL) use. Until today, however, CL solution manufacturing protocols lack testing of anti-amoebic activity. This study investigates the effectiveness of CL solutions available on the Dutch market against trophozoites and cysts of and . Sixteen CL solutions were tested: 13 multiple purpose solutions (MPS), 2 hydrogen peroxidase solutions (HPS) and 1 povidone-iodine-based solution (PIS). The Spearman-Karber (SK) log reduction method and an XTT colorimetric assay were used to evaluate the effectiveness at the manufacturer's minimum recommended disinfection time (MMRDT) and after eight hours. At the MMRDT, one MPS showed an SK mean log reduction (MLR) of >3.0 against trophozoites. Two additional MPS and both HPS reached this threshold after eight hours. The SK MLR values for trophozoites were between 1 and 3 at all time points. Using the XTT colorimetric assay, only HPS 1 showed >99.9% reduction (equivalent to 3 log reduction) in metabolic activity of trophozoites after eight hours. For , both HPS and PIS showed a metabolic reduction of >99.9% after eight hours. Cysts were resistant against all solutions. We conclude that following the manufacturer's guidelines, few solutions provide sufficient effectiveness against trophozoites and none against cysts. The results underline the importance of adequate hygiene when handling CLs.
角膜炎几乎都与隐形眼镜（CL）的使用有关。然而，直到如今，隐形眼镜护理液的生产协议仍缺乏抗阿米巴活性测试。本研究调查了荷兰市场上在售的隐形眼镜护理液对 和 的滋养体及包囊的有效性。测试了16种隐形眼镜护理液：13种多用途护理液（MPS）、2种过氧化氢护理液（HPS）和1种聚维酮碘基护理液（PIS）。采用Spearman-Karber（SK）对数减少法和XTT比色法，在制造商推荐的最短消毒时间（MMRDT）及8小时后评估其有效性。在MMRDT时，一种MPS对 滋养体的SK平均对数减少率（MLR）>3.0。另外两种MPS和两种HPS在8小时后达到该阈值。在所有时间点， 滋养体的SK MLR值在1至3之间。使用XTT比色法，仅HPS 1在8小时后使 滋养体的代谢活性降低>99.9%（相当于3个对数减少）。对于 ，两种HPS和PIS在8小时后均显示代谢降低>99.9%。包囊对所有护理液均有抗性。我们得出结论，按照制造商的指导方针，很少有护理液对 滋养体有足够的有效性，对包囊则均无效。结果强调了处理隐形眼镜时保持良好卫生的重要性。
