Zardetto-Smith A M, Gray T S
Department of Anatomy, Loyola University Medical Center, Maywood, IL 60153.
Neurosci Lett. 1987 Sep 23;80(2):163-6. doi: 10.1016/0304-3940(87)90647-1.
A direct neuronal projection from the nucleus of the solitary tract (NTS) to the subfornical organ (SFO) in rats was demonstrated using the Phaseolus vulgaris leucoagglutinin (PHA-L) anterograde tracing technique. Small deposits of PHA-L were iontophoretically placed within the caudal parts of the nucleus of the solitary tract. Brain sections were processed for visualization of PHA-L using the avidin-biotin immunoperoxidase technique. PHA-L immunoreactive beaded axons were observed within the subependymal and central parts of the SFO. The results demonstrate an anatomical substrate for direct feedback from cardiovascular regions of the NTS to the SFO. This pathway may be important for relaying blood pressure and associated body fluid volume changes to the SFO.
采用菜豆白细胞凝集素（PHA-L）顺行示踪技术，证实了大鼠孤束核（NTS）至穹窿下器官（SFO）存在直接的神经元投射。将PHA-L的小沉积物通过离子电渗法置于孤束核的尾部。使用抗生物素蛋白-生物素免疫过氧化物酶技术对脑切片进行处理，以观察PHA-L。在穹窿下器官的室管膜下和中央部分观察到了PHA-L免疫反应性的串珠状轴突。结果表明，从孤束核的心血管区域到穹窿下器官存在直接反馈的解剖学基础。该通路对于将血压及相关的体液量变化传递至穹窿下器官可能具有重要意义。
