Lokhov Petr G, Balashova Elena E, Trifonova Oxana P, Maslov Dmitry L, Archakov Alexander I
Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, Russia.
Pharmaceutics. 2023 Feb 16;15(2):661. doi: 10.3390/pharmaceutics15020661.
In omics sciences, many compounds are measured simultaneously in a sample in a single run. Such analytical performance opens up prospects for improving cellular cancer vaccines and other cell-based immunotherapeutics. This article provides an overview of proteomics technology, known as cell proteomic footprinting. The molecular phenotype of cells is highly variable, and their antigenic profile is affected by many factors, including cell isolation from the tissue, cell cultivation conditions, and storage procedures. This makes the therapeutic properties of cells, including those used in vaccines, unpredictable. Cell proteomic footprinting makes it possible to obtain controlled cell products. Namely, this technology facilitates the cell authentication and quality control of cells regarding their molecular phenotype, which is directly connected with the antigenic properties of cell products. Protocols for cell proteomic footprinting with their crucial moments, footprint processing, and recommendations for the implementation of this technology are described in this paper. The provided footprints in this paper and program source code for their processing contribute to the fast implementation of this technology in the development and manufacturing of cell-based immunotherapeutics.
在组学科学中，一次运行就能在一个样本中同时测量多种化合物。这种分析性能为改进细胞癌症疫苗和其他基于细胞的免疫疗法开辟了前景。本文概述了一种称为细胞蛋白质组足迹分析的蛋白质组学技术。细胞的分子表型高度可变，其抗原谱受许多因素影响，包括从组织中分离细胞、细胞培养条件和储存程序。这使得细胞的治疗特性，包括用于疫苗的细胞，变得不可预测。细胞蛋白质组足迹分析使得获得可控的细胞产品成为可能。也就是说，这项技术有助于对细胞进行鉴定，并根据其分子表型对细胞进行质量控制，而分子表型与细胞产品的抗原特性直接相关。本文描述了细胞蛋白质组足迹分析的方案及其关键步骤、足迹处理以及该技术实施的建议。本文提供的足迹以及用于处理它们的程序源代码有助于该技术在基于细胞的免疫疗法的开发和生产中快速实施。
