Borawska-Jarmułowicz Barbara, Mastalerczuk Grażyna
Department of Agronomy, Institute of Agriculture, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159 St., 02-776 Warsaw, Poland.
Plants (Basel). 2023 Feb 17;12(4):910. doi: 10.3390/plants12040910.
Drought and allelopathic conditions impact the germination of seeds of grass and legume species used in mixtures on grassland. This study evaluated the effects of drought and root extracts at different pH levels on the germination and characteristics of seedlings of and selected species. Two experiments were carried out: the first explored the effects of drought induced by PEG solutions (0.0, -0.3, and -0.6 MPa) under silicon (Si) application; the second studied the influence of root extracts (12 and 24 g/100 mL HO) and variable pH solutions (control, 5.0, and 6.5) on seed germination. Germination was carried out on Petri dishes for individual species and in two-species mixtures. The drought conditions did not affect the germination capacity of seeds, but it increased the dry weight of the seedlings of the tested species. The application of silicon decreased the root length of the seedlings of all species, independent of the germination conditions. The higher concentration of root extract combined with the studied pH solutions had an inhibitive impact on the germination capacity of . Our findings revealed that the lower concentration of root extract had a beneficial effect on the morphological features (length of roots and leaves, fresh and dry weight) of the seedlings when germination was carried out separately for both species. In the mixture, the effect was especially marked for (only in terms of the root length and fresh weight of seedlings). The applied root extracts in combination with the acidic pH conditions limited the germination capacity and growth of the seedlings of the most when germination was performed separately. It was concluded from this study that silicon application did not improve the germination capacity of seeds under drought conditions, whereas the germination of seeds in a mixture with mitigated the negative allelopathic effects of root extracts on the seed germination capacity and morphological features of seedlings of .
干旱和化感条件会影响用于草地混播的禾本科和豆科植物种子的萌发。本研究评估了干旱和不同pH值的根系提取物对所选物种种子萌发及幼苗特性的影响。进行了两项实验：第一项实验探究了在施用硅的情况下，PEG溶液（0.0、-0.3和-0.6 MPa）诱导的干旱的影响；第二项实验研究了根系提取物（12和24 g/100 mL H₂O）和可变pH值溶液（对照、5.0和6.5）对种子萌发的影响。种子萌发在培养皿中针对单个物种以及两种物种的混合物进行。干旱条件并未影响种子的萌发能力，但增加了受试物种幼苗的干重。施用硅降低了所有物种幼苗的根长，与萌发条件无关。较高浓度的根系提取物与所研究的pH值溶液相结合，对[物种名称]的萌发能力产生了抑制作用。我们的研究结果表明，当分别对两种物种进行萌发时，较低浓度的根系提取物对幼苗的形态特征（根长和叶长、鲜重和干重）具有有益影响。在混合物中，这种影响对[物种名称]尤为显著（仅就幼苗的根长和鲜重而言）。当分别进行萌发时，所施用的根系提取物与酸性pH条件相结合，对[物种名称]幼苗的萌发能力和生长限制最大。从本研究得出的结论是，在干旱条件下施用硅并不能提高种子的萌发能力，而[物种名称]与[物种名称]混合播种时，[物种名称]的种子萌发减轻了[物种名称]根系提取物对[物种名称]种子萌发能力和幼苗形态特征的负面化感作用。
