Schiavi Daniele, Ronchetti Riccardo, Di Lorenzo Veronica, Vivani Riccardo, Giovagnoli Stefano, Camaioni Emidio, Balestra Giorgio M
Department of Agriculture and Forest Sciences (DAFNE), University of Tuscia, Via San Camillo de Lellis snc, 01100 Viterbo, Italy.
Department of Pharmaceutical Sciences (DSF), University of Perugia, Via del Liceo 1, 06123 Perugia, Italy.
Plants (Basel). 2023 Feb 18;12(4):939. doi: 10.3390/plants12040939.
Nanotechnology is rapidly gaining ground in crop protection, with the growing quest for sustainable nanopesticides and nanocarriers for plant pathogen management. Among them, cellulose nanocrystals (CNC) are emerging as innovative agrofood-waste-derived antimicrobial materials. In this work, new chemical and enzymatic CNC extraction methods from tomato harvest residues were evaluated. The obtained nanomaterials were characterized and tested for their antimicrobial properties on pv. (Pto), the causal agent of bacterial speck disease on tomato. Both protocols were efficient. The enzymatic extraction method was greener, producing purer CNC at slightly lower yield. The obtained CNC, although they weakly inhibited cell growth and did not promote reactive oxygen species (ROS) formation, provoked bacterial aggregation and the inhibition of biofilm production and swimming motility. Both protocols produced CNC with similar morpho-chemical features, as well as promising antimicrobial activity against plant bacterial pathogens, suggesting their potential role in sustainable crop protection strategies. The new protocols could be a valuable alternative to conventional methods.
纳米技术在作物保护领域正迅速发展，人们对用于植物病原体管理的可持续纳米农药和纳米载体的需求日益增长。其中，纤维素纳米晶体（CNC）正成为一种创新的源自农业食品废弃物的抗菌材料。在这项工作中，评估了从番茄收获残余物中提取新型化学和酶法纤维素纳米晶体的方法。对所获得的纳米材料进行了表征，并测试了它们对番茄细菌性斑点病病原菌丁香假单胞菌番茄致病变种（Pto）的抗菌性能。两种方法都很有效。酶法提取方法更环保，能以略低的产率生产出更纯净的纤维素纳米晶体。所获得的纤维素纳米晶体虽然对细胞生长的抑制作用较弱且不促进活性氧（ROS）的形成，但能引起细菌聚集，并抑制生物膜形成和游动性。两种方法生产的纤维素纳米晶体具有相似的形态化学特征，以及对植物细菌病原体有前景的抗菌活性，表明它们在可持续作物保护策略中的潜在作用。这些新方法可能是传统方法的一个有价值的替代方案。
