Danesh-Doust Mohammad, Irajirad Rasoul, Vaziri Nezamdoust Fereshteh, Khademi Sara, Montazerabadi Alireza
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Cell J. 2023 Feb 1;25(2):126-134. doi: 10.22074/cellj.2022.557552.1068.
Increasing research has been focused on the development of various nanocomplexes as targeted contrast media in diagnostic modalities, mainly in computed tomography (CT) scan imaging. Herein, we report a new method that uses Triptorelin [a luteinizing hormone-releasing hormone (LHRH) agonist]-targeted gold nanoparticles (AuNPs) via alginate for early detection of cancer by molecular CT imaging.
In the experimental study, the formed multifunctional AuNPs coated with alginate conjugated with Triptorelin peptide (Triptorelin-Alginate-AuNPs) were synthesized and characterized via different techniques, including transmission electron microscopy (TEM), dynamic light scattering (DLS), and fourier transform infrared (FTIR) spectroscopy. The MTT assay was applied to calculate the toxicity of the NPs.
The results indicated that the formed Triptorelin-Alginate-AuNPs with an Au core size of ~18 nm are noncytotoxic at 127-, 254-, 381- and 508-mM concentrations and revealed significant improvement in the attenuation of X-rays intensity and contrast to noise ratio (CNR), compared with non-targeted cells at the highest energies (90, 120, 140 kVp). At 90 kVp, compared to non-targeted cells, targeted cells (Triptorelin-Alginate-AuNPs) enable 1.58, 1.69, 3.7 and 3.43 times greater contrast at a concentration of 127 mM, 254 mM, 381 mM, and 508 mM, respectively.
These results suggest that the developed Triptorelin-Alginate-AuNPs may be considered an effective contrast agent for molecular CT imaging of gonadotropin-releasing hormone (GnRH) receptor-expressing cancer cells.
越来越多的研究聚焦于开发各种纳米复合物作为诊断模式中的靶向造影剂，主要用于计算机断层扫描（CT）成像。在此，我们报告一种新方法，即通过海藻酸盐使用曲普瑞林[促黄体激素释放激素（LHRH）激动剂]靶向金纳米颗粒（AuNPs），用于通过分子CT成像早期检测癌症。
在实验研究中，合成了包覆有与曲普瑞林肽共轭的海藻酸盐的多功能AuNPs（曲普瑞林-海藻酸盐-AuNPs），并通过不同技术进行表征，包括透射电子显微镜（TEM）、动态光散射（DLS）和傅里叶变换红外（FTIR）光谱。应用MTT法计算纳米颗粒的毒性。
结果表明，形成的Au核尺寸约为18 nm的曲普瑞林-海藻酸盐-AuNPs在127 mM、254 mM、381 mM和508 mM浓度下无细胞毒性，并且与最高能量（90、120、140 kVp）下的非靶向细胞相比，X射线强度衰减和对比噪声比（CNR）有显著改善。在90 kVp时，与非靶向细胞相比，靶向细胞（曲普瑞林-海藻酸盐-AuNPs）在127 mM、254 mM、381 mM和508 mM浓度下的对比度分别提高1.58倍、1.69倍、3.7倍和3.43倍。
这些结果表明，所开发的曲普瑞林-海藻酸盐-AuNPs可被视为用于表达促性腺激素释放激素（GnRH）受体的癌细胞分子CT成像的有效造影剂。
