Department of Physics, Chuo University, Tokyo, Japan.
Nano Life Science Institute, Kanazawa University, Kanazawa, Japan.
Methods Mol Biol. 2023;2646:109-124. doi: 10.1007/978-1-0716-3060-0_10.
The stator unit of the bacterial flagellar motor coordinates the number of active stators in the motor by sensing changes in external load and ion motive force across the cytoplasmic membrane. The structural dynamics of the stator unit at the single-molecule level is key to understanding the sensing mechanism and motor assembly. High-speed atomic force microscopy (HS-AFM) is a powerful tool for directly observing dynamically acting biological molecules with high spatiotemporal resolution without interfering with their function. Here, we describe protocols for single-molecule imaging of the Na-driven MotPS stator complex by HS-AFM.
细菌鞭毛马达的定子单元通过感应细胞质膜两侧外部负载和离子动力的变化来协调马达中活性定子的数量。了解感应机制和马达组装的关键是定子单元在单分子水平上的结构动力学。高速原子力显微镜（HS-AFM）是一种强大的工具，可在不干扰其功能的情况下，以高时空分辨率直接观察动态作用的生物分子。在这里，我们描述了通过 HS-AFM 对 Na 驱动的 MotPS 定子复合物进行单分子成像的方案。
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