使用两种互补方法对围植入期小母牛血清进行定量蛋白质组学分析。
Quantitative proteomic analysis of heifer serum at the peri-implantation period using two complementary methods.
作者信息
Ruiz Álvarez Jimena Inés, Teijeiro Juan Manuel, Evans Caroline, Marini Patricia Estela
机构信息
Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; and Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Buenos Aires, Argentina.
出版信息
Reprod Fertil Dev. 2023 Mar;35(6):416-421. doi: 10.1071/RD22228.
CONTEXT
Systemic events resulting from mother-embryo relation at the peri-implantation period may result in specific changes to the protein composition of serum and thus provide a source of biomarkers for early detection of pregnancy.
AIMS
We set out to use two different quantitative proteomic approaches to test this hypothesis by comparing heifer serum at the peri-implantation period to that from cycling heifers.
METHODS
Two-dimensional electrophoresis (2-DE) and isobaric tags were used for relative and absolute quantitation (iTRAQ) of proteins.
KEY RESULTS
These methods yielded complementary data indicating biomarker candidate proteins.
CONCLUSIONS
Different proteomic methods provide different and complementary information that needs to be analysed in order to consider proteins as potential biomarkers.
IMPLICATIONS
In order to characterise the proteome under specific conditions, the use of complementary techniques is advisable.
背景
着床前期母胎关系引发的全身事件可能导致血清蛋白质组成发生特定变化，从而为早期妊娠检测提供生物标志物来源。
目的
我们着手采用两种不同的定量蛋白质组学方法，通过比较着床前期小母牛血清与发情周期小母牛血清来验证这一假设。
方法
二维电泳（2-DE）和等压标签相对与绝对定量（iTRAQ）用于蛋白质定量。
关键结果
这些方法产生了互补数据，表明了生物标志物候选蛋白。
结论
不同的蛋白质组学方法提供不同且互补的信息，为将蛋白质视为潜在生物标志物，需要对这些信息进行分析。
启示
为了在特定条件下表征蛋白质组，建议使用互补技术。
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