Heck Kabre L, Walters Lauren M, Kunze Madeline L, Calderón Angela I
Department of Drug Discovery and Development, Harrison School of Pharmacy, Auburn University, Auburn AL 36849, United States.
J Food Compost Anal. 2023 May;118. doi: 10.1016/j.jfca.2023.105155. Epub 2023 Jan 19.
Mart., commonly known as açaí, is a fruit that grows on a palm tree native to the Amazon region. Quantitation of bioactive constituents is a crucial preliminary step before utilizing extracts for biological assays so they may be normalized and administered according to a specific constituent concentration. Açaí has four main anthocyanin analytes: cyanidin 3-glucoside, cyanidin 3-sambubioside, cyanidin 3-rutinoside, and peonidin 3-rutinoside. This is the first comparison of açaí anthocyanin profiles between fresh fruits, processed powders, and botanical dietary supplement capsules. The materials examined shared a similar anthocyanin profile, with cyanidin 3-rutinoside being the most abundant (0.380 ± 0.006 - 15.1 ± 0.01 mg/g), followed by cyanidin 3-glucoside (0.0988 ± 0.0031 - 8.95 ± 0.01 mg/g). Among the botanical dietary supplement capsules, the two formulations varied greatly in anthocyanin concentration despite both being aqueous extracts (0.650 ± 0.011 - 0.924 ± 0.010 mg/g versus 1.23 ± 0.01 - 1.27 ± 0.02 mg/g). Previous LC-MS methods range from 35-120 min per injection, while we report a 10 min quantitative method for analysis of anthocyanins in various açaí materials that is fast, reproducible, and accurate. The method produced is useful to assure the quality, efficacy and safety of food and dietary supplement materials containing açaí.
玛氏果，通常称为阿萨伊果，是一种生长在原产于亚马逊地区的棕榈树上的水果。在利用提取物进行生物测定之前，对生物活性成分进行定量是关键的初步步骤，这样它们就可以标准化并根据特定成分浓度进行给药。阿萨伊果有四种主要的花青素分析物：矢车菊素3-葡萄糖苷、矢车菊素3-桑布双糖苷、矢车菊素3-芸香糖苷和芍药素3-芸香糖苷。这是首次对新鲜水果、加工粉末和植物性膳食补充剂胶囊中的阿萨伊果花青素谱进行比较。所检测的材料具有相似的花青素谱，其中矢车菊素3-芸香糖苷含量最高（0.380±0.006 - 15.1±0.01毫克/克），其次是矢车菊素3-葡萄糖苷（0.0988±0.0031 - 8.95±0.01毫克/克）。在植物性膳食补充剂胶囊中，尽管两种制剂都是水提取物，但花青素浓度差异很大（0.650±0.011 - 0.924±0.010毫克/克与1.23±0.01 - 1.27±0.02毫克/克）。以前的液相色谱-质谱方法每次进样时间为35 - 120分钟，而我们报告了一种10分钟的定量方法，用于分析各种阿萨伊果材料中的花青素，该方法快速、可重复且准确。所建立的方法有助于确保含有阿萨伊果的食品和膳食补充剂材料的质量、功效和安全性。
