School of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, P. R. China.
Phytochem Anal. 2021 May;32(3):395-403. doi: 10.1002/pca.2986. Epub 2020 Oct 11.
The bark of Ilex rotunda (BIr) has been used as a traditional Chinese medicine for a long time in China. Triterpene saponins (TSs) were considered to be the major bioactive ingredients and possessed antiplatelet aggregation activity. Identification of TSs in BIr is a necessary step to understand the biological properties.
This article describes a method for the rapid identification of TSs in BIr.
An analytical method based on high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-QTOF-MS/MS) was established to characterise the TSs in BIr. Chromatographic separation was performed on an Agilent poroshell 120 EC-C18 column with gradient elution using water containing 0.1% (v/v) formic acid and acetonitrile as the mobile phase.
A total of 26 TSs were identified or tentatively identified from BIr. Of them, 23 TSs were first reported from BIr.
This was the first comprehensive investigation on TSs in BIr, and the results formed the foundation for the further development and utilisation of BIr in the future.
冬青属植物的树皮在中国长期以来一直被用作传统中药。三萜皂苷（TSs）被认为是主要的生物活性成分，具有抗血小板聚集活性。鉴定 BIr 中的 TSs 是了解其生物特性的必要步骤。
本文描述了一种快速鉴定 BIr 中 TSs 的方法。
建立了一种基于高效液相色谱-电喷雾离子化-四极杆飞行时间串联质谱（HPLC-ESI-QTOF-MS/MS）的分析方法，用于鉴定 BIr 中的 TSs。色谱分离在 Agilent poroshell 120 EC-C18 柱上进行，采用梯度洗脱，流动相为含有 0.1%（v/v）甲酸的水和乙腈。
从 BIr 中鉴定或初步鉴定出 26 种 TSs。其中，23 种 TSs 首次从 BIr 中报道。
这是对 BIr 中 TSs 的首次全面研究，为今后 BIr 的进一步开发利用奠定了基础。
