Phimnuan Preeyawass, Dirand Zélie, Tissot Marion, Worasakwutiphong Saran, Sittichokechaiwut Anuphan, Grandmottet François, Viyoch Jarupa, Viennet Céline
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000 Thailand.
UMR 1098 RIGHT INSERM EFS FC, DImaCell Imaging Resource Center, University of Franche-Comté, Besançon 25000 France.
ACS Omega. 2023 Feb 7;8(7):6813-6824. doi: 10.1021/acsomega.2c07507. eCollection 2023 Feb 21.
In diabetic patients, the process of wound healing is usually delayed or impaired. A diabetic environment could be associated with dermal fibroblast dysfunction, reduced angiogenesis, the release of excessive proinflammatory cytokines, and senescence features. Alternative therapeutic treatments using natural products are highly demanded for their high potential of bioactive activity in skin repair. Two natural extracts were combined to develop fibroin/aloe gel wound dressing. Our previous studies revealed that the prepared film enhances the healing rate of diabetic foot ulcers (DFUs). Moreover, we aimed to explore its biological effects and underlying biomolecular mechanisms on normal dermal, diabetic dermal, and diabetic wound fibroblasts. Cell culture experiments showed that the γ-irradiated blended fibroin/aloe gel extract film promotes skin wound healing by enhancing cell proliferation and migration, vascular epidermal growth factor (VEGF) secretion, and cell senescence prevention. Its action was mainly linked to the activation of the mitogen-activated protein kinases/extracellular signal-regulated kinase (MAPK/ERK) signaling pathway known to regulate various cellular activities, including proliferation. Therefore, the findings of this study confirm and support our previous data. The blended fibroin/aloe gel extract film displays a biological behavior with favorable properties for delayed wound healing and can be considered as a promising therapeutic approach in the treatment of diabetic nonhealing ulcers.
在糖尿病患者中，伤口愈合过程通常会延迟或受损。糖尿病环境可能与真皮成纤维细胞功能障碍、血管生成减少、促炎细胞因子过度释放以及衰老特征有关。由于天然产物在皮肤修复中具有很高的生物活性潜力，因此对使用天然产物的替代治疗方法有很高的需求。将两种天然提取物结合起来开发了丝素蛋白/芦荟凝胶伤口敷料。我们之前的研究表明，制备的薄膜可提高糖尿病足溃疡（DFU）的愈合率。此外，我们旨在探讨其对正常真皮、糖尿病真皮和糖尿病伤口成纤维细胞的生物学效应及其潜在的生物分子机制。细胞培养实验表明，经γ射线辐照的丝素蛋白/芦荟凝胶混合提取物薄膜通过增强细胞增殖和迁移、血管内皮生长因子（VEGF）分泌以及预防细胞衰老来促进皮肤伤口愈合。其作用主要与丝裂原活化蛋白激酶/细胞外信号调节激酶（MAPK/ERK）信号通路的激活有关，已知该信号通路可调节包括增殖在内的各种细胞活动。因此，本研究的结果证实并支持了我们之前的数据。丝素蛋白/芦荟凝胶混合提取物薄膜表现出对延迟伤口愈合具有良好特性的生物学行为，可被视为治疗糖尿病难愈合溃疡的一种有前景的治疗方法。
