Dittmar Maria, Weissenböck Florian Peter, Rentmeister Andrea
Institut für Biochemie, Westfälische Wilhelms-Universität Münster, Corrensstraße 36, D-48149 Münster, Deutschland.
Biospektrum (Heidelb). 2023;29(1):31-34. doi: 10.1007/s12268-023-1881-3. Epub 2023 Feb 21.
Messenger RNA (mRNA) shows great potential for medical applications, as recently demonstrated by the mRNA-based vaccines against the coronavirus. In addition, it has long been used for ectopic gene expression in cells and model organisms. While numerous methodologies are available for controlling gene expression at the level of transcription, approaches to control translation are scarce. Here we review strategies for direct light-mediated activation of mRNA translation via photocleavable groups and their potential to achieve spatial and temporal control of protein production.
信使核糖核酸（mRNA）在医学应用中显示出巨大潜力，最近基于mRNA的抗冠状病毒疫苗就证明了这一点。此外，它长期以来一直用于细胞和模式生物中的异位基因表达。虽然有许多方法可用于在转录水平控制基因表达，但控制翻译的方法却很少。在这里，我们综述了通过光可裂解基团直接光介导激活mRNA翻译的策略及其实现蛋白质生产时空控制的潜力。
