Nicoletti Carolina F, Roschel Hamilton, Merege-Filho Carlos, Lima Alisson P, Gil Saulo, Pinhel Marcela A S, Noronha Natalia Y, Santo Marco A, Jacome Amalia, Crujeiras Ana B, Gualano Bruno, Nonino Carla B
Department of Internal Medicine, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão preto, Brazil.
Applied Physiology & Nutrition Research Group, School of Physical Educaton and Sport, Rheumatology Division, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao paulo, Brazil.
Front Sports Act Living. 2023 Feb 8;5:1092050. doi: 10.3389/fspor.2023.1092050. eCollection 2023.
Exercise training and bariatric surgery have been shown to independently modulate DNA methylation profile in clusters of genes related to metabolic and inflammatory pathways. This study aimed to investigate the effects of a 6-month exercise training program on DNA methylation profile in women who underwent bariatric surgery. In this exploratory, quasi-experimental study, we analyzed DNA methylation levels by array technology in eleven women who underwent Roux-en-Y Gastric Bypass and a 6-month, three-times-a-week, supervised exercise training program. Epigenome Wide Association Analysis showed 722 CpG sites with different methylation level equal to or greater than 5% ( < 0.01) after exercise training. Some of these CpGs sites were related to pathophysiological mechanisms of inflammation, specially Th17 cell differentiation (FDR value < 0.05 and < 0.001). Our data showed epigenetic modification in specific CpG sites related to Th17 cell differentiation pathway in post-bariatric women following a 6-months exercise training program.
运动训练和减肥手术已被证明可独立调节与代谢和炎症途径相关的基因簇中的DNA甲基化谱。本研究旨在调查为期6个月的运动训练计划对接受减肥手术的女性DNA甲基化谱的影响。在这项探索性的准实验研究中，我们通过阵列技术分析了11名接受Roux-en-Y胃旁路手术并参加了为期6个月、每周三次的有监督运动训练计划的女性的DNA甲基化水平。表观基因组全关联分析显示，运动训练后有722个CpG位点的甲基化水平差异等于或大于5%（<0.01）。其中一些CpG位点与炎症的病理生理机制有关，特别是Th17细胞分化（FDR值<0.05和<0.001）。我们的数据显示，在为期6个月的运动训练计划后，减肥后女性中与Th17细胞分化途径相关的特定CpG位点发生了表观遗传修饰。
