Zhao Yankun, Shao Wei, Wang Fulan, Ma Jiaoxiao, Chen He, Wang Shuai, Wu Yating, Wang Cheng, Zheng Nan, Wang Jiaqi, Liu Huimin
Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, People's Republic of China.
Ministry of Agriculture and Rural Affairs Laboratory of Quality and Safety Risk Assessment of Agro-Products, Urumqi 830091, People's Republic of China.
J Vet Res. 2022 Dec 16;66(4):581-590. doi: 10.2478/jvetres-2022-0069. eCollection 2022 Dec.
is an important zoonotic pathogen that affects milk production and quality and poses a threat to public health. Treatment of infections with this bacterium exploits antimicrobials, to which the resistance of is a growing problem. Addressing the possibility of a correlation between this pathogen's genetic factors for antimicrobial resistance and virulence, this study attempted to identify the relevant genes.
Antimicrobial resistance of isolated from 497 Chinese bovine mastitic milk samples was detected by the broth microdilution method. Eight drug resistance genes and eleven virulence genes were detected using PCR.
was 100% susceptible to rifampicin and vancomycin, 93.33% susceptible to sulfisoxazole and sulfamethoxazole, but 100% resistant to ≥3 of the 16 antimicrobial agents, thereby being multidrug resistant, with resistance to oxacillin, tetracycline, erythromycin, clindamycin, and gentamicin being common. The , and genes were carried by 73.33%, 66.67% and 60.00% of the strains, respectively. The carriage rates of the , , , and virulence genes were greater than 40%, and were not observed in any strain, and +++++ combined virulence gene patterns were the most commonly detected.
Antimicrobial resistance of is still a great concern for cattle health in China, and multidrug resistance coupled with the high positive rates of this bacterium's strains for virulence genes indicates the importance of surveillance and susceptibility tests.
是一种重要的人畜共患病原体，会影响牛奶产量和质量，并对公众健康构成威胁。用抗菌药物治疗该细菌感染，但该细菌对这些抗菌药物的耐药性问题日益严重。为探讨该病原体的抗菌耐药性遗传因素与毒力之间的相关性，本研究试图鉴定相关基因。
采用肉汤微量稀释法检测从497份中国奶牛乳腺炎乳样中分离出的的抗菌药物敏感性。使用PCR检测8种耐药基因和11种毒力基因。
对利福平和万古霉素的敏感性为100%，对磺胺异恶唑和磺胺甲恶唑的敏感性为93.33%，但对16种抗菌药物中的≥3种耐药率为100%，因此具有多重耐药性，对苯唑西林、四环素、红霉素、克林霉素和庆大霉素耐药较为常见。、和基因分别存在于73.33%、66.67%和60.00%的菌株中。、、、和毒力基因的携带率均大于40%，在任何菌株中均未检测到，+++++联合毒力基因模式最为常见。
在中国，对奶牛健康来说，的抗菌药物耐药性仍是一个重大问题，多重耐药性加上该细菌菌株毒力基因的高阳性率表明监测和药敏试验的重要性。
