Dela Justina Vanessa, de Freitas Raiany Alves, Arishe Olufunke O, Giachini Fernanda R, Webb R Clinton, Priviero Fernanda
Graduate Program in Biological Sciences, Federal University of Goias, Goiânia, Goias, Brazil.
Department of Cell Biology and Anatomy-School of Medicine, University of South Carolina, Columbia, SC, United States.
Front Physiol. 2023 Feb 10;14:998951. doi: 10.3389/fphys.2023.998951. eCollection 2023.
Piezo1 channel is a sensor for shear-stress in the vasculature. Piezo1 activation induces vasodilation, and its deficiency contributes to vascular disorders, such as hypertension. In this study, we aimed o determine whether Piezo1 channel has a functional role in the dilation of pudendal arteries and corpus cavernosum (CC). For this, male Wistar rats were used, and the relaxation of the pudendal artery and CC was obtained using the Piezo1 activator, Yoda1, in the presence and absence of Dooku (Yoda1 antagonist), GsMTx4 (non-selective mechanosensory channel inhibitor) and L-NAME (nitric oxide synthase inhibitor). In the CC, Yoda1 was also tested in the presence of indomethacin (non-selective COX inhibitor) and tetraethylammonium (TEA, non-selective potassium channel inhibitor). The expression of Piezo1 was confirmed by Western blotting. Our data show that Piezo1 activation leads to the relaxation of the pudendal artery and CC as the chemical activator of Piezo1, Yoda1, relaxed the pudendal artery (47%) and CC (41%). This response was impaired by L-NAME and abolished by Dooku and GsMTx4 in the pudendal artery only. Indomethacin and TEA did not affect the relaxation induced by Yoda1 in the CC. Limited tools to explore this channel prevent further investigation of its underlying mechanisms of action. In conclusion, our data demonstrate that Piezo1 is expressed and induced the relaxation of the pudendal artery and CC. Further studies are necessary to determine its role in penile erection and if erectile dysfunction is associated with Piezo1 deficiency.
Piezo1通道是脉管系统中剪切应力的传感器。Piezo1激活可诱导血管舒张，其功能缺陷会导致诸如高血压等血管疾病。在本研究中，我们旨在确定Piezo1通道在阴部动脉和海绵体（CC）舒张中是否具有功能性作用。为此，我们使用了雄性Wistar大鼠，并在存在和不存在杜库（Yoda1拮抗剂）、GsMTx4（非选择性机械感觉通道抑制剂）和L - NAME（一氧化氮合酶抑制剂）的情况下，使用Piezo1激活剂Yoda1来获得阴部动脉和CC的舒张情况。在CC中，还在存在吲哚美辛（非选择性COX抑制剂）和四乙铵（TEA，非选择性钾通道抑制剂）的情况下对Yoda1进行了测试。通过蛋白质印迹法确认了Piezo1的表达。我们的数据表明，Piezo1激活导致阴部动脉和CC舒张，因为Piezo1的化学激活剂Yoda1使阴部动脉舒张了47%，使CC舒张了41%。这种反应在L - NAME存在时受损，仅在阴部动脉中被杜库和GsMTx4消除。吲哚美辛和TEA不影响Yoda1在CC中诱导的舒张。用于探索该通道的工具有限，阻碍了对其潜在作用机制的进一步研究。总之，我们的数据表明Piezo1表达并诱导了阴部动脉和CC的舒张。需要进一步研究以确定其在阴茎勃起中的作用，以及勃起功能障碍是否与Piezo1缺陷有关。
