Gong Liu'e, Ying Shumin, Zhang Yafen, Wang Jiaoyu, Sun Guochang
College of Life Science, China Jiliang University, Hangzhou 310018, Zhejiang, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Feb 25;39(2):459-471. doi: 10.13345/j.cjb.220618.
Plant diseases and insect pests threaten the safety of crop production greatly. Traditional methods for pest management are challenged by the problems such as environmental pollution, off-target effects, and resistance of pathogens and insects. New biotechnology-based strategies for pest control are expected to be developed. RNA interference (RNAi) is an endogenous process of gene regulation, which has been widely used to study the gene functions in various organisms. In recent years, RNAi-based pest management has received increasing attention. The effective delivery of the exogenous interference RNA into the targets is a key step in RNAi-mediated plant diseases and pest control. Considerable advances were made on the mechanism of RNAi, and various RNA delivery systems were developed for efficient pest control. Here we review the latest advances on mechanisms and influencing factors of RNA delivery, summarize the strategies of exogenous RNA delivery in RNAi-mediated pest control, and highlight the advantages of nanoparticle complexes in dsRNA delivery.
植物病虫害对作物生产安全构成极大威胁。传统的害虫管理方法面临着环境污染、非靶标效应以及病原体和昆虫抗性等问题的挑战。人们期望开发基于新生物技术的害虫防治策略。RNA干扰（RNAi）是一种内源性基因调控过程，已被广泛用于研究各种生物体中的基因功能。近年来，基于RNAi的害虫管理受到越来越多的关注。将外源性干扰RNA有效递送至靶标是RNAi介导的植物病虫害防治的关键步骤。在RNAi机制方面取得了相当大的进展，并且开发了各种RNA递送系统以实现高效的害虫防治。在此，我们综述了RNA递送机制和影响因素的最新进展，总结了RNAi介导的害虫防治中外源RNA递送的策略，并强调了纳米颗粒复合物在双链RNA递送中的优势。
