Division of Molecular Entomology, Department of Zoology, School of Life Sciences, Periyar University, Salem, 636011, India.
Division of Genomic Resources, ICAR-National Bureau of Agricultural Insect Resources, H.A. Farm Post, Hebbal, P.B. No. 2491, Bangalore, 560024, India.
Mol Biol Rep. 2024 Feb 24;51(1):355. doi: 10.1007/s11033-023-09187-6.
Nanoparticle-based delivery systems have emerged as powerful tools in the field of pest management, offering precise and effective means of delivering double-stranded RNA (dsRNA), a potent agent for pest control through RNA interference (RNAi). This comprehensive review aims to evaluate and compare various types of nanoparticles for their suitability in dsRNA delivery for pest management applications. The review begins by examining the unique properties and advantages of different nanoparticle materials, including clay, chitosan, liposomes, carbon, gold and silica. Each material's ability to protect dsRNA from degradation and its potential for targeted delivery to pests are assessed. Furthermore, this review delves into the surface modification strategies employed to enhance dsRNA delivery efficiency. Functionalization with oligonucleotides, lipids, polymers, proteins and peptides is discussed in detail, highlighting their role in improving stability, cellular uptake, and specificity of dsRNA delivery.This review also provides valuable guidance on choosing the most suitable nanoparticle-based system for delivering dsRNA effectively and sustainably in pest management. Moreover, it identifies existing knowledge gaps and proposes potential research directions aimed at enhancing pest control strategies through the utilization of nanoparticles and dsRNA.
纳米粒子基递药系统已成为害虫管理领域的有力工具,为通过 RNA 干扰(RNAi)来控制害虫提供了精确有效的双链 RNA(dsRNA)传递手段。本综述旨在评估和比较各种类型的纳米粒子在 dsRNA 传递方面用于害虫管理应用的适用性。本综述首先考察了不同纳米粒子材料(包括粘土、壳聚糖、脂质体、碳、金和二氧化硅)的独特性质和优势。评估了每种材料保护 dsRNA 免受降解的能力及其将 dsRNA 靶向递送至害虫的潜力。此外,本综述深入探讨了用于提高 dsRNA 传递效率的表面修饰策略。详细讨论了与寡核苷酸、脂质、聚合物、蛋白质和肽的功能化,强调了它们在提高 dsRNA 传递稳定性、细胞摄取和特异性方面的作用。本综述还就如何选择最适合的基于纳米粒子的系统以有效和可持续地在害虫管理中传递 dsRNA 提供了有价值的指导。此外,它还确定了现有知识空白,并提出了通过利用纳米粒子和 dsRNA 增强害虫控制策略的潜在研究方向。
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