Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Wesley University of Science and Technology, Ondo City, Nigeria.
Mol Biol Rep. 2020 Aug;47(8):6309-6319. doi: 10.1007/s11033-020-05666-2. Epub 2020 Jul 22.
Climate change imposes a great threat to world food security and encourages insect pest proliferation and spreading. Because of these challenges, identifying novel biotechnology pest management and its applications is inevitable. RNA interference (RNAi) is a gene regulatory process used for the maintenance and regulation of host defences against invading viruses. Nevertheless, it is widely used for the analysis of gene function. In recent years, the potential use of RNA interference (RNAi) as a tool for manipulating crop traits, as well as an alternative for crop protection, has undergone outstanding developments. In this review, we describe some genes involved in insect dsRNA uptake and discuss the reasons for varying RNAi response in insect pests, emphasizing the presence of nucleases and double-stranded RNA binding protein. We explore recent breakthroughs in innovative dsRNA delivery for efficient and effective knockdown in insect pests. Conclusively, topical delivery of dsRNA combined with a nanoparticle complex holds great potential for RNAi-mediated pest control.
气候变化对世界粮食安全构成了巨大威胁，促使昆虫害虫大量繁殖和传播。由于这些挑战，必须寻找新的生物技术害虫管理及其应用。RNA 干扰 (RNAi) 是一种用于维持和调节宿主防御入侵病毒的基因调控过程。然而，它被广泛用于分析基因功能。近年来，RNA 干扰 (RNAi) 作为一种操纵作物特性的工具以及作物保护的替代手段已经取得了显著的发展。在这篇综述中，我们描述了一些参与昆虫 dsRNA 摄取的基因，并讨论了昆虫对 RNAi 反应不同的原因，强调了核酸酶和双链 RNA 结合蛋白的存在。我们探讨了在昆虫中高效有效 RNAi knockdown 的创新 dsRNA 传递方面的最新突破。总之，dsRNA 的局部递送与纳米颗粒复合物相结合，为 RNAi 介导的害虫防治提供了巨大的潜力。
