College of Health Science and Engineering, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Wuhan Business University, Wuhan 430056, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jun 5;294:122503. doi: 10.1016/j.saa.2023.122503. Epub 2023 Feb 16.
Inflammation is a critical physiological process in the human body, which is closely related to numerous disorders and cancers. ONOO is generated and functionalized in the inflamed process, but the roles of ONOO are still blurred. To illuminate the roles of ONOO, we fabricated an intramolecular charge transfer (ICT)-based fluorescence probe, HDM-Cl-PN, for the ratiometric determination of ONOO in the inflamed mouse model. The probe displayed a gradual fluorescence increase at 676 nm and a fluorescence drop at 590 nm toward 0-10.5 μM ONOO, and the ratio of 676 nm fluorescence and 590 nm fluorescence varied from 0.7 to 24.7. The significantly changed ratio and favorable selectivity ensure the sensitive detection of subtle changes in cellular ONOO. Thanks to the excellent sensing performance, HDM-Cl-PNin vivo ratiometrically visualized ONOO fluctuations in the LPS-triggered inflammatory process. Overall, this work not only expatiated the rational design for a ratiometric ONOO probe but also built a bridge to investigate the connections between ONOO and inflammation in living mice.
炎症是人体中一种关键的生理过程，与许多疾病和癌症密切相关。ONOO 在炎症过程中生成并发挥作用，但 ONOO 的作用仍不清楚。为了阐明 ONOO 的作用，我们设计了一种基于分子内电荷转移（ICT）的荧光探针 HDM-Cl-PN，用于在炎症小鼠模型中对 ONOO 进行比率测定。该探针对 0-10.5 μM ONOO 的响应表现为 676nm 处荧光逐渐增强和 590nm 处荧光逐渐减弱，676nm 荧光与 590nm 荧光的比值在 0.7 到 24.7 之间变化。显著变化的比值和良好的选择性确保了对细胞内 ONOO 细微变化的灵敏检测。由于出色的传感性能，HDM-Cl-PN 在体内能够对 LPS 触发的炎症过程中的 ONOO 波动进行比率成像。总的来说，这项工作不仅阐述了比率型 ONOO 探针的合理设计，还为研究活鼠体内 ONOO 与炎症之间的联系搭建了桥梁。
