College of Science, Nanjing Forestry University, Nanjing, 210037, China.
Department of Pharmacy, Shandong Medical College, 5460 Erhuannanlu Road, Jinan, 250002, China.
J Biol Inorg Chem. 2022 Sep;27(6):595-603. doi: 10.1007/s00775-022-01954-3. Epub 2022 Aug 17.
For ratiometrically imaging peroxynitrite (ONOO) in living cells, we devised and fabricated a novel fluorescent nanoprobe, NC-NP, in this study. To achieve ratiometric fluorescence response towards ONOO, NC-NP used 3-(2-benzothiazolyl) coumarin (Cou-Bz) as the internal reference and 1,8-naphthimide derivative (Naph-PN) as a fluorescent ONOO probe. These compounds were incorporated into an amphiphilic block polymer called Pluronic F-127. In addition to an ultrafast response to ONOO, NC-NP also showed great selectivity and sensitive detection (detection limit was 4.51 μM). It was important to note that NC-NP demonstrated solid performance for ONOO fluorescence ratio imaging in living cells, highlighting its potential for ONOO-related chemical biology research.
为了在活细胞中对过氧亚硝酸盐（ONOO）进行比率成像，我们在这项研究中设计并制备了一种新型荧光纳米探针 NC-NP。为了实现对 ONOO 的比率荧光响应，NC-NP 采用 3-(2-苯并噻唑基)香豆素（Cou-Bz）作为内参，1,8-萘酰亚胺衍生物（Naph-PN）作为荧光 ONOO 探针。这些化合物被掺入一种称为 Pluronic F-127 的两亲嵌段聚合物中。除了对 ONOO 的超快响应外，NC-NP 还表现出出色的选择性和灵敏检测（检测限为 4.51 μM）。值得注意的是，NC-NP 在活细胞中对 ONOO 荧光比率成像表现出良好的性能，突出了其在与 ONOO 相关的化学生物学研究中的应用潜力。
