Qiao Xuejiao, Fu Chenkun, Chen Yizhen, Fang Fang, Zhang Yaoyu, Ding Lingyun, Yang Kai, Pan Baozhu, Xu Nan, Yu Ke, Tao Huchun, Zhang Lijuan
Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, Guangdong, China.
College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen 518118, Guangdong, China.
Bioresour Technol. 2023 Apr;374:128784. doi: 10.1016/j.biortech.2023.128784. Epub 2023 Feb 26.
It has been widely reported that fluoroquinolones (FQs) can affect the anaerobic ammonium oxidization (anammox) microorganisms, which interferes with the performance of nitrogen removal from wastewater. However, the metabolic mechanism of anammox microorganisms responding to FQs has rarely been explored. In this study, it was found that 20 μg/L FQs promoted the nitrogen removal performance of anammox microorganisms in batch exposure assays, and 36-51% of FQs were removed simultaneously. Combined metabolomics and genome-resolved metagenomic analysis revealed up-regulated carbon fixation in anammox bacteria (AnAOB), while purine and pyrimidine metabolism, protein generation and transmembrane transport were enhanced in AnAOB and symbiotic bacteria by 20 μg/L FQs. Consequently, hydrazine dehydrogenation, nitrite reduction, and ammonium assimilation were bolstered, improving the nitrogen removal efficiency of the anammox system. These results revealed the potential roles of specific microorganisms in response to emerging FQs and provided further information for practical application of anammox technology in wastewater treatment.
已有广泛报道称，氟喹诺酮类药物（FQs）会影响厌氧氨氧化（anammox）微生物，进而干扰废水脱氮性能。然而，anammox微生物对FQs响应的代谢机制鲜有研究。本研究发现，在批次暴露试验中，20 μg/L的FQs可促进anammox微生物的脱氮性能，同时36% - 51%的FQs被同步去除。代谢组学和基因组解析宏基因组分析相结合表明，anammox菌（AnAOB）中的碳固定上调，而20 μg/L的FQs使AnAOB和共生细菌中的嘌呤和嘧啶代谢、蛋白质生成及跨膜运输增强。因此，联氨脱氢、亚硝酸盐还原和氨同化得到加强，提高了anammox系统的脱氮效率。这些结果揭示了特定微生物对新兴FQs响应的潜在作用，并为anammox技术在废水处理中的实际应用提供了更多信息。
