宿主越多，不选择宿主范围更广的噬菌体。
More's the Same-Multiple Hosts Do Not Select for Broader Host Range Phages.
机构信息
Department of Biology and Toxicology, Ashland University, Ashland, OH 44805, USA.
Blood Bank, Akron City Hospital, Akron, OH 44304, USA.
出版信息
Viruses. 2023 Feb 13;15(2):518. doi: 10.3390/v15020518.
Bacteriophage host range is a result of the interactions between phages and their hosts. For phage therapy, phages with a broader host range are desired so that a phage can infect and kill the broadest range of pathogen strains or related species possible. A common, but not well-tested, belief is that using multiple hosts during the phage isolation will make the isolation of broader host range phage more likely. Using a group system, we compared the host ranges of phages isolated on one or four hosts and found that there was no difference in the breadth of host ranges of the isolated phages. Both narrow and broader host range phage were also equally likely to be isolated from either isolation procedure. While there are methods that reliably isolate broader host range phages, such as sequential host isolation, and there are other reasons to use multiple hosts during isolation, multiple hosts are not a consistent way to obtain broader host range phages.
噬菌体的宿主范围是噬菌体与其宿主之间相互作用的结果。对于噬菌体疗法，人们希望噬菌体具有更广泛的宿主范围，以便噬菌体能够感染和杀死尽可能广泛的病原体菌株或相关物种。一种常见但未经充分验证的观点是，在噬菌体分离过程中使用多种宿主会使分离更广泛宿主范围的噬菌体的可能性更大。使用分组系统，我们比较了在一个或四个宿主上分离的噬菌体的宿主范围，发现分离的噬菌体的宿主范围没有差异。无论是窄宿主范围噬菌体还是更广泛宿主范围噬菌体，都同样有可能从两种分离程序中分离出来。虽然有一些方法可以可靠地分离更广泛宿主范围的噬菌体，例如顺序宿主分离，但也有其他原因需要在分离过程中使用多种宿主，但是使用多种宿主并不是获得更广泛宿主范围噬菌体的可靠方法。
Zotero 插件