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用焯烫辣木叶粉替代豆粕对似野结鱼(Bleeker,1850)幼鱼生长性能和血液学反应的影响

Growth performance and hematological responses of silver barb ( bleeker, 1850) fingerlings to dietary blanched moringa ( lam.) leaf meal as a substitute of soybean meal.

作者信息

Farhad Farhabun Binte, Hashem Shaharior, Rana K M Shakil, Salam M A

机构信息

Department of Aquaculture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

出版信息

Heliyon. 2023 Feb 7;9(2):e13552. doi: 10.1016/j.heliyon.2023.e13552. eCollection 2023 Feb.

Abstract

The fastest-growing aquaculture industry relies heavily on animal protein, fishmeal and plant protein to maintain production levels. Therefore, present study was conducted to perceive the effectiveness of blanched moringa () leaf meal (MLM) as a replacement of soybean meal in silver barb () fingerling diet. Four experimental feeds were prepared replacing soybean meal with MLM at 0, 10, 30, and 50%. Fish were reared for 60 days in 12 hapas installed in three similar size and shaped ponds comprising four treatments each having 3 replications. Fish were fed with the experimental diet at 5% body weight twice daily. Fish growth parameters and length-weight relationship were assessed. To determine their resistance to stressful conditions, the fish were submerged in low pH-5 solution at the conclusion of the experiment. According to the results of the fish growth metrics, the majority of the parameters were comparable and statistically insignificant between the treatments. However, when compared to the control, T and T treatments, the T treatment demonstrated increased survival, PER, and fish production. In addition, other parameters such as percent weight gain, SGR, FCR and FCE were higher in control but statistically similar with T. Besides, the length-weight relationship of silver barb fingerlings fed with all the test diets showed a positive association and isometric growth pattern. With the incremental addition of MLM to the fish diet, the hematological parameters-red blood cells (RBC), white blood cells (WBC), hemoglobin (Hb), and mean corpuscular hemoglobin (MCH) gradually increased. The fish fed the T diet had the highest recorded stress tolerance (6.50 ± 0.50 min), whereas the fish on the control diet had the lowest (T, 4.77 ± 0.68 min). According to the study, MLM has the potential to replace soybean meal in the diet of silver barb fingerlings to the tune of 50:50 without having an adverse impact on growth. It can enhance fish hematological performance and tolerance for unfavorable environmental conditions as well.

摘要

发展最快的水产养殖业严重依赖动物蛋白、鱼粉和植物蛋白来维持生产水平。因此,本研究旨在探讨热烫辣木叶粉(MLM)替代倒刺鲃鱼种饲料中豆粕的效果。制备了四种实验饲料,分别用0%、10%、30%和50%的MLM替代豆粕。鱼在安装于三个大小和形状相似的池塘中的12个围网中饲养60天,每个池塘包含四种处理,每种处理有3个重复。鱼每天按体重的5%投喂两次实验饲料。评估了鱼的生长参数和体长 - 体重关系。为了确定它们对压力条件的抵抗力,在实验结束时将鱼浸入pH值为5的低溶液中。根据鱼生长指标的结果,大多数参数在各处理之间具有可比性且无统计学显著差异。然而,与对照组、T1和T2处理相比,T3处理的存活率、蛋白质效率比(PER)和鱼产量有所提高。此外,其他参数如体重增加百分比、特定生长率(SGR)、饲料系数(FCR)和饲料转化率(FCE)在对照组中较高,但与T3在统计学上相似。此外,投喂所有测试饲料的倒刺鲃鱼种的体长 - 体重关系呈正相关且为等速生长模式。随着鱼饲料中MLM添加量的增加,血液学参数——红细胞(RBC)、白细胞(WBC)、血红蛋白(Hb)和平均红细胞血红蛋白(MCH)逐渐增加。投喂T3饲料的鱼记录到的应激耐受性最高(6.50 ± 0.50分钟),而投喂对照饲料的鱼最低(T1,4.77 ± 0.68分钟)。根据研究,MLM有潜力以50:50的比例替代倒刺鲃鱼种饲料中的豆粕,且不会对生长产生不利影响。它还可以提高鱼的血液学性能和对不利环境条件的耐受性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a727/9958432/0b202bbb46d6/gr1.jpg

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