Lee Min Seo, Park Mi-Jeong, Son Purena, Rho Taekeun, Kwon Kae Kyoung, Park Jin-Sook
Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, 385, Haeyang-ro, Yeongdo-gu, Busan, 49111, Republic of Korea.
Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34054, Republic of Korea.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006834.
A novel bacterium, designated strain 2305UL40-4, was isolated from (a Korean limpet) collected from Ulleung Island, Republic of Korea. The cells were Gram-stain-negative, aerobic, rod-shaped (0.5-0.9 µm×1.5-2.2 µm) and non-motile. Growth was observed at 22-36 °C (optimum 25-30 °C), pH 6-8 (optimum pH 7) and in the presence of 0.5-4.0% (w/v, optimum 2.0%) NaCl. The 16S rRNA gene sequence analysis revealed that strain 2305UL40-4 shared 97.4% similarity with DBTF-13. The average nucleotide identity value between the two strains was 76.5%, and the digital DNA-DNA hybridization value was 19.0%. Both values are well below the species delineation thresholds. The DNA G+C content of strain 2305UL40-4 was 63.8 mol%. The major fatty acids were C, C and summed feature 8 (C c and/or C c). Major polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, five unidentified aminolipids and fifteen unidentified lipids. Ubiquinone-10 was the predominant quinone. The new strain differs from in its ability to utilize d-raffinose, d-ribose and d-xylose. Based on these polyphasic taxonomic characteristics, strain 2305UL40-4 is proposed as a novel species within the genus , with the name sp. nov. The type strain is 2305UL40-4 (=KCCM 43505=MCCC 1K09164).
从韩国郁陵岛采集的（一种韩国帽贝）中分离出一种新型细菌，命名为2305UL40-4菌株。细胞革兰氏染色阴性，需氧，杆状（0.5 - 0.9 µm×1.5 - 2.2 µm），无运动性。在22 - 36 °C（最适25 - 30 °C）、pH 6 - 8（最适pH 7）以及0.5 - 4.0%（w/v，最适2.0%）NaCl条件下可生长。16S rRNA基因序列分析表明，2305UL40-4菌株与DBTF-13的相似性为97.4%。两菌株间的平均核苷酸同一性值为76.5%，数字DNA-DNA杂交值为19.0%。这两个值均远低于物种划分阈值。2305UL40-4菌株的DNA G+C含量为63.8 mol%。主要脂肪酸为C、C以及总和特征8（C c和/或C c）。主要极性脂包括磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酰胆碱、5种未鉴定的氨基脂和15种未鉴定的脂。泛醌-10是主要醌类。新菌株在利用d-棉子糖、d-核糖和d-木糖的能力上与（某菌株）不同。基于这些多相分类学特征，提议将2305UL40-4菌株作为（某属）内的一个新物种，命名为（新物种名称）sp. nov.。模式菌株为2305UL40-4（=KCCM 43505=MCCC 1K09164）。
