严重急性呼吸综合征冠状病毒2（SARS-CoV-2）负链亚基因组RNA中共有发夹环结构的鉴定。
Identification of consensus hairpin loop structure among the negative sense subgenomic RNAs of SARS-CoV-2.
作者信息
Bokolia Naveen Prakash, Gadepalli Ravisekhar
机构信息
Viral Research and Diagnostic Laboratory, Microbiology Department, All India Institute of Medical Sciences, Jodhpur, 342001 India.
出版信息
Bull Natl Res Cent. 2023;47(1):28. doi: 10.1186/s42269-023-01002-3. Epub 2023 Feb 23.
BACKGROUND
SARS-CoV-2 is the causative agent of worldwide pandemic disease coronavirus disease 19. SARS-CoV-2 bears positive sense RNA genome that has organized and complex pattern of replication/transcription process including the generation of subgenomic RNAs. Transcription regulatory sequences have important role in the pausing of replication/transcription and generation of subgenomic RNAs.
RESULTS
In the present bioinformatics analysis, a consensus secondary structure was identified among negative sense subgenomic RNAs of SARS-CoV-2. This consensus region is present at the adjacent of initiation codon.
CONCLUSIONS
This study proposed that consensus structured domain could involve in mediating the long pausing of replication/transcription complex and responsible for subgenomic RNA production.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1186/s42269-023-01002-3.
背景
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是全球大流行疾病冠状病毒病19的病原体。SARS-CoV-2携带正链RNA基因组，其复制/转录过程具有有组织且复杂的模式，包括亚基因组RNA的产生。转录调控序列在复制/转录的暂停以及亚基因组RNA的产生中起重要作用。
结果
在本生物信息学分析中，在SARS-CoV-2的负链亚基因组RNA中鉴定出一个共有二级结构。该共有区域存在于起始密码子附近。
结论
本研究提出，共有结构域可能参与介导复制/转录复合体的长时间暂停，并负责亚基因组RNA的产生。
补充信息
在线版本包含可在10.1186/s42269-023-01002-3获取的补充材料。
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