Nakamura S, Serikawa T, Yamakawa K, Nishida S, Kozaki S, Sakaguchi G
Microbiol Immunol. 1978;22(10):591-6. doi: 10.1111/j.1348-0421.1978.tb00409.x.
All of the 8 strains that were previously assumed to be nontoxigenic Clostridium botulinum type C were re-examined for their toxigenicity and were demonstrated by trypsinization of the culture filtrates to produce C2 toxin under improved cultural conditions. One per cent glucose added to trypticase peptone medium enhanced C2 toxin production. The larger the spore population, the higher the C2 toxicity and when spore population was smaller than a level of 10(4)/ml, no C2 toxicity was demonstrated. The C2 toxin was produced only during sporulation and not during vegetative growth.
对先前被认为是非产毒型C型肉毒梭菌的所有8个菌株重新进行产毒性检测，结果表明，在改良培养条件下，通过对培养滤液进行胰蛋白酶处理，这些菌株可产生C2毒素。在胰蛋白酶蛋白胨培养基中添加1%的葡萄糖可提高C2毒素的产量。孢子数量越多，C2毒性越高；当孢子数量低于10⁴/ml时，未显示出C2毒性。C2毒素仅在孢子形成过程中产生，而在营养生长过程中不产生。
