职业性接触二硫化碳工人口腔颊黏膜细胞 DNA 损伤。
DNA Damage on Buccal Epithelial Cells, Personal Working in the Rubber Industry Occupationally Exposed to Carbon Disulfide (CS2).
机构信息
Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, Karnataka, India.
Department of Biotechnology, Selvam Arts and Science College, Namakkal, Tamilnadu, India.
出版信息
Asian Pac J Cancer Prev. 2023 Feb 1;24(2):357-361. doi: 10.31557/APJCP.2023.24.2.357.
INTRODUCTION
The most significant industrial utilization of carbon disulfide (CS2) has been in the manufacture of cellulose rayon, cellophane, and rubber industry. CS2 prompts expanded recurrence of chromosomal variations in laborers occupationally exposed to CS2.
MATERIALS AND METHODS
In the current study, the DNA analysis was carried out from exfoliated buccal epithelial cells from rubber industry workers exposed to CS2 and an equal number of healthy control subjects. Both the control and experimental subjects were categorized by their smoking habits such as smokers (S) and non-smokers (NS). Furthermore, experimental subjects were further separated based on their exposure period. Students t-test statistical tools were used to analyze the final results.
RESULTS
The present analysis identified a high frequency of DNA damage in rubber industry workers (16.55±0.43) than control subjects (9.8±0.21). Also, maximum number of DNA damage detected in smoking experimental group (18.27±0.02) than non-smoking experimental (15.02±0.01) and smoking control groups (10.25±0.04 ).
CONCLUSION
Smoking habits synergistically increased the DNA damage in the rubber industry workers exposed to CS2.
简介
二硫化碳（CS2）最重要的工业用途是制造纤维素纤维、赛璐珞和橡胶工业。CS2 会导致职业接触 CS2 的工人染色体变异的发生率增加。
材料和方法
在本研究中，对接触 CS2 的橡胶工业工人和等量健康对照者的口腔上皮细胞进行了 DNA 分析。对照和实验对象均根据吸烟习惯进行分类，如吸烟者（S）和非吸烟者（NS）。此外，实验对象还根据暴露期进一步分开。使用学生 t 检验统计工具来分析最终结果。
结果
目前的分析表明，橡胶工业工人的 DNA 损伤频率高于对照组（16.55±0.43 比 9.8±0.21）。此外，在吸烟实验组中检测到的 DNA 损伤数量最多（18.27±0.02），而非吸烟实验组（15.02±0.01）和吸烟对照组（10.25±0.04）。
结论
吸烟习惯与接触 CS2 的橡胶工业工人的 DNA 损伤呈协同作用。
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