Vincent Helen A, Henderson Charlotte A, Lopes Cardoso Daniela, Callaghan Anastasia J
School of Biological Sciences and Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth, UK.
Methods Mol Biol. 2023;2633:163-184. doi: 10.1007/978-1-0716-3004-4_13.
RNA performs a wide variety of vital cellular functions. These functions typically require interactions with other biological macromolecules, often as part of an intricate communication network. High-throughput techniques capable of analyzing RNA-based interactions are therefore essential. Functional-RNA arrays address this need, providing the capability of performing hundreds of miniature assays in parallel. Here we describe a method to generate functional-RNA arrays using in vitro transcription of a DNA template array and in situ RNA capture. We also suggest how functional-RNA arrays could be applied to investigating RNA-RNA interactions.
RNA执行多种重要的细胞功能。这些功能通常需要与其他生物大分子相互作用，这往往是复杂通讯网络的一部分。因此，能够分析基于RNA的相互作用的高通量技术至关重要。功能性RNA阵列满足了这一需求，具备并行进行数百种微型检测的能力。在此，我们描述了一种利用DNA模板阵列的体外转录和原位RNA捕获来生成功能性RNA阵列的方法。我们还提出了功能性RNA阵列可如何应用于研究RNA-RNA相互作用。
