优化的活细胞成像方案用于研究斑马鱼中免疫细胞与β细胞之间由营养过剩引发的相互作用。
Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish.
机构信息
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA; Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, No. 301 Middle Yanchang Road, Shanghai 200072, China.
出版信息
STAR Protoc. 2023 Mar 17;4(1):102039. doi: 10.1016/j.xpro.2022.102039. Epub 2023 Jan 17.
Here, we provide an optimized protocol to observe the interactions between infiltrating immune cells and islet β cells using live imaging. This protocol is useful for the characterization of cell-cell interactions and for the direct visualization of immune cell migration to the principal pancreatic islet during islet inflammation. We describe the preparation of zebrafish transgenic lines and detail steps for setting up the fish for live confocal imaging. For more details on the use and execution of this protocol, please refer to Yang et al. (2022)..
在这里，我们提供了一个优化的方案，使用活体成像来观察浸润性免疫细胞与胰岛β细胞之间的相互作用。该方案可用于研究细胞-细胞相互作用，并直接观察免疫细胞在胰岛炎症过程中向主胰岛的迁移。我们描述了斑马鱼转基因系的制备，并详细介绍了活体共聚焦成像的设置步骤。如需了解本方案的更多详细信息，请参考 Yang 等人（2022 年）的研究。
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