阿根廷科尔多瓦风疹病毒分离株中存在中和结构域。
Presence of a neutralizing domain in isolates of rubella virus in Cordoba, Argentina.
作者信息
Cordoba P, Grutadauria S L, Cuffini C, Zapata M T
机构信息
Instituto de Virología J. M. Vanella, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
出版信息
Clin Diagn Lab Immunol. 1997 Jul;4(4):493-5. doi: 10.1128/cdli.4.4.493-495.1997.
We studied the presence of a neutralizing epitope of rubella virus (RV) in locally circulating strains in Cordoba, Argentina, using binding by the monoclonal antibody (MAb) H3. This epitope is contained in a sequence of the E1 glycoprotein (E1208-239) represented by the synthetic peptide SP15. H3 MAb showed specific binding to SP15 by enzyme-linked immunosorbent assay (ELISA). One wild-type postnatal isolate, four clones derived from this isolate, and one congenital isolate were reactive with H3 by ELISA. These results suggest that the region of RV represented by SP15 is a domain present in locally circulating strains.
我们使用单克隆抗体（MAb）H3的结合作用，研究了阿根廷科尔多瓦当地流行毒株中是否存在风疹病毒（RV）的中和表位。该表位包含在由合成肽SP15代表的E1糖蛋白序列（E1208 - 239）中。通过酶联免疫吸附测定（ELISA），H3单克隆抗体显示出与SP15的特异性结合。一株野生型产后分离株、从该分离株衍生的四个克隆株以及一株先天性分离株通过ELISA与H3发生反应。这些结果表明，由SP15代表的RV区域是当地流行毒株中存在的一个结构域。
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