Benassi F O, Palmieri de Morey S, Leardini N, Aguilera I N, Martín Talavera B, Lo Sardo M
Centro de Investigación y Desarrollo Tecnológico, Facultad de Ciencias Exactas, Universidad Nacional de Misiones, Argentina.
Rev Argent Microbiol. 1986;18(2):79-82.
The investigation was focused on the occurrence of different species and biovarieties of fecal streptococci in Zaiman creek waters, the basin of which comprises important zones of Posadas, Misiones. The samples were collected from points near a meat-packing industrial establishment which pours its effluents in the above mentioned water course. So far, 18 sampling operations have been carried out in representative geographic locations. The methodology followed for detecting and characterizing the present species consisted in an enrichment procedure performed by incubating samples in Azida Dextrose broth at 35 degrees C during 24-48 h. Further streaks were made on KF Agar and Kanamycin Agar plates. The biochemical study of the colonies developed in Brain Heart Agar media was the one described in the American Water Standards (APHA and EPA). The isolation frequency of the different species of fecal streptococci is shown. (Table 2). The efficiency of both culture media KF Agar and Kanamycin Aesculin Azide Agar proved to be similar. (Table 1). The distribution of fecal streptococci by sampling points shows that the pollution comes not only from the pourings of effluents but also from the population settlement near the water course.
该调查聚焦于扎伊曼溪流水域中粪链球菌不同种类和生物变种的出现情况,该流域涵盖米西奥内斯省波萨达斯市的重要区域。样本采集于一家肉类加工企业附近的地点,该企业将废水排入上述水道。到目前为止,已在具有代表性的地理位置进行了18次采样作业。检测和鉴定现有物种所采用的方法包括富集程序,即将样本在叠氮葡萄糖肉汤中于35摄氏度孵育24 - 48小时。随后在KF琼脂平板和卡那霉素琼脂平板上进行划线。在脑心琼脂培养基上生长的菌落的生化研究采用了美国水标准(美国公共卫生协会和美国环境保护局)中描述的方法。展示了不同种类粪链球菌的分离频率(表2)。结果证明KF琼脂和卡那霉素七叶苷叠氮琼脂这两种培养基的效率相似(表1)。按采样点划分的粪链球菌分布表明,污染不仅来自废水排放,还来自水道附近的居民点。