改良卡那霉素-七叶苷-叠氮化物琼脂以提高水样中粪链球菌计数的选择性。
Modification of kanamycin-esculin-azide agar to improve selectivity in the enumeration of fecal streptococci from water samples.
作者信息
Audicana A, Perales I, Borrego J J
机构信息
Laboratorio de Salud Pública, Delegación Territorial de Sanidad de Vizcaya, Bilbao Paris Vasco, Spain.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4178-83. doi: 10.1128/aem.61.12.4178-4183.1995.
Abstract
Kanamycin-esculin-azide agar was modified by increasing the concentration of sodium azide to 0.4 g liter-1 and replacing kanamycin sulfate with 5 mg of oxolinic acid liter-1. The modification, named oxolinic acid-esculin-azide (OAA) agar, was compared with Slanetz-Bartley and KF agars by using drinking water and seawater samples. The OAA agar showed higher specificity, selectivity, and recovery efficiencies than those obtained by using the other media. In addition, no confirmation of typical colonies was needed when OAA agar was used, which significantly shortens the time of sample processing and increases the accuracy of the method.
摘要
卡那霉素-七叶苷-叠氮化物琼脂通过将叠氮化钠浓度提高到0.4克/升并将硫酸卡那霉素替换为5毫克/升的恶喹酸进行改良。这种改良后的培养基被命名为恶喹酸-七叶苷-叠氮化物（OAA）琼脂，通过使用饮用水和海水样本，将其与Slanetz-Bartley琼脂和KF琼脂进行比较。结果显示，与使用其他培养基相比，OAA琼脂具有更高的特异性、选择性和回收率。此外，使用OAA琼脂时无需对典型菌落进行确认，这显著缩短了样本处理时间并提高了方法的准确性。
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