Department of Mechanical Engineering, University of Washington, Seattle, Washington, USA; email:
Department of Laboratory Medicine and Pathology, University of Washington, Seattle, Washington, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2023 Jun 14;16(1):231-252. doi: 10.1146/annurev-anchem-091222-092734. Epub 2023 Feb 28.
In recent years, there has been a revived appreciation for the importance of spatial context and morphological phenotypes for both understanding disease progression and guiding treatment decisions. Compared with conventional 2D histopathology, which is the current gold standard of medical diagnostics, nondestructive 3D pathology offers researchers and clinicians the ability to visualize orders of magnitude more tissue within their natural volumetric context. This has been enabled by rapid advances in tissue-preparation methods, high-throughput 3D microscopy instrumentation, and computational tools for processing these massive feature-rich data sets. Here, we provide a brief overview of many of these technical advances along with remaining challenges to be overcome. We also speculate on the future of 3D pathology as applied in translational investigations, preclinical drug development, and clinical decision-support assays.
近年来，人们重新认识到空间背景和形态表型对于理解疾病进展和指导治疗决策的重要性。与传统的 2D 组织病理学相比，3D 病理学是目前医学诊断的金标准，它为研究人员和临床医生提供了在其自然体积背景下可视化数量级更多组织的能力。这得益于组织制备方法的快速发展、高通量 3D 显微镜仪器以及用于处理这些大规模特征丰富数据集的计算工具。在这里，我们简要概述了其中的许多技术进步以及有待克服的剩余挑战。我们还推测了 3D 病理学在转化研究、临床前药物开发和临床决策支持检测中的未来应用。
